Investigating the Immunomodulation Potential of Different Drug Combinations of Andrographis paniculata and Tinospora cordifolia Methanolic Extracts | Chapter 2 | Pharmaceutical Science: New Insights and Developments Vol. 6

Background: A good number of herbal drugs are known to possess immunomodulatory properties and generally act by stimulating both specific and nonspecific immunity. The plants, Andrographis paniculata  (Kalmegh) and Tinospora cordifolia (Giloya), are becoming increasingly popular for a variety of diseases and infectious conditions, primarily influencing the host defence mechanism.

 

Objective: This study mainly focuses on the immunomodulatory effect of different drug combinations (DC-I to DC-V) of methanolic extracts of aerial parts of Andrographis paniculata  (AP) and mature stems of Tinospora cordifolia (TC).

 

Materials and Methods: The Aerial parts of Andrographis paniculata  and selected mature stems of the Tinospora cordifolia were collected. The coarse powder (40-mesh) of shade-dried aerial parts of AP and mature stems of TC (500g each) was subjected separately to successive extraction with 1000 mL each of petroleum ether (60-80oC) followed by methanol. The doses of DC-I to DC-V were selected and were administered orally at doses of 200 mg/kg body weight to Albino Wistar rats and compared with the control group and standard drug, i.e. cyclophosphamide (100mg/kg), respectively. The animals were randomly selected, marked to permit individual identification and kept in the cages for 7 days prior to dosing to allow for acclimatisation to the laboratory conditions. Results obtained were statistically analysed by using One-way ANOVA followed by Dunnett’s comparison test.

 

Results: The time-dependent effect of different Drug Combinations (DC-I to DC-V) on morphometric functional changes in mice (peritoneal macrophages) was evaluated. Results suggest that although all five tested combinations (DC-I to DC-V) prepared using different proportions of methanol extracts of A. paniculata and T. cordifolia (100:Zero, 75:25, 50:50, 25:75 and Zero:100) showed a significant increase in macrophase count, neutrophil adhesion (NA), haemagglutinating antibody (HA) titre and delayed type hypersensitivity (DTH) response. In rats immunised with sheep RBC, DC-I to DC-V enhanced the humoral antibody response to the antigen and significantly potentiated the cellular immunity by facilitating the footpad thickness response to sheep RBC in sensitised rats on chosen experimental models.

 

Conclusion: The values of macrophase, NA, HA, DTH and cellular responses of combination DC-II at a 200 mg/kg body weight were statistically significant as compared to other combinations. Combination DC-II exhibited the best immunostimulant potential, indicating that it has promising immunomodulatory activity, as proposed from a commercial point of view. Immunostimulant therapy may be beneficial for patients under a variety of settings that include prevention and treatment of various infectious diseases, warranting further investigation in future studies.

Author (s) Details

 

Yadav R
Department of Pharmacy, MAJU, Rampur, U.P., India.

 

Yadav N
SRMS, College of Engg. and Tech., (Pharmacy), Bareilly, U.P., India.

 

Kharya M D
Department of Pharmaceutical Sciences, Dr. H. S. Gour Central University, Sagar, M.P., India

 

Please see the book here:- https://doi.org/10.9734/bpi/psnid/v6/5395

Effect of Tinospora Cordifolia Leaf Extract Concentrations on Optical and Photocatalytic Properties of Green Synthesized Silver Nanoparticles| Chapter 9 | New Frontiers in Physical Science Research Vol. 6

 Silver nanoparticles (AgNPs) have happened combined by way of green route utilizing Tinospora cordifolia (Giloe) leaf extract and studies of the ocular and photocatalytic features of combined white nanoparticles have been completed activity. The composition of AgNPs was resolved at various response occasions by UV-Vis spectrometer. The difference of piece content of combined AgNPs accompanying various concentrations of giloe extract was intentional and it is noticed that particle intensity increases accompanying the increase in the aggregation of giloe extract. The ocular band break strength of AgNPs was determined by utilizing Tauc’s connection and the difference of band breach strength accompanying the aggregation of plant extract was intentional. With the decrease in concentration of Giloi leaf extract, the ocular strength band break of AgNPs increases, that signifies a decrease in atom proportion. The green combined AgNPs operated as a good impetus for the depravity of Methylene Blue (MB) dye unprotected to seeable light. The MB dye disgraced to almost 90% inside 60 notes of uncovering opportunity.

Author(s) Details:

Suvankar Saha,
Department of Physics, Bodoland University, Kokrajhar, Assam-783370, India.

P. K. Mochahari,
Department of Physics, Bodoland University, Kokrajhar, Assam-783370, India.

Please see the link here: https://stm.bookpi.org/NFPSR-V6/article/view/9215

Green Synthesis of Silver Nanoparticles Using Azadirachta Indica and Tinospora Cordifolia Leaves Extracts- Their Optical and Photocatalytic Study| Chapter 5 | Research Aspects in Chemical and Materials Sciences Vol. 4

 Silver nanoparticles (AgNPs) have happened synthesised via green route utilizing two different plant extracts: Azadirachta indica (Neem) and Tinospora cordifolia (Giloe) leaves extracts. The composition of AgNPs has been noticed from the colour change of the solution and is further habitual by UV-Vis Spectroscopy. Optical properties of synthesized samples have happened carried out utilizing UV–VIS spectrophotometer. For Ag- A. indica NPs, the absorbance maximum occurs at 440 nm and the equivalent absorbance peak occurred at 345 nm in the case of Ag- T. cordifolia NPs. Optical band breach energy has existed estimated utilizing Tauc’s relation and differences of band gap strength at different opportunity intervals were analysed for the AgNPs samples able using Azadirachta indica (Neem) as well asTinospora cordifolia (Giloe) leaves extracts. Particle capacity has been establish to decrease with opportunity for Ag- A. indica NPs whereas piece size increases accompanying time for Ag- T. cordifolia NPs. The combined Ag NPs act as a incentive for the degradation of methylene vulgar (MB) dye under visible light. The portion of degradation was found expected 87.56% within 45 notes of exposure to apparent light.

Author(s) Details:

Suvankar Saha,
Department of Physics, Bodoland University, Kokrajhar, Assam-783370, India.

P. K. Mochahari,
Department of Physics, Bodoland University, Kokrajhar, Assam-783370, India.

Please see the link here: https://stm.bookpi.org/RACMS-V4/article/view/8692