Aims: The purpose of this study is to evaluate the pro-oxidant and antioxidant properties of ascorbic acid (AA) in Musca domestica larvae.
Study Design: The study design involved
assessing the pro-oxidant properties of M.
domestica larvae by exposing them to different dosages of AA. Additionally,
the antioxidant capacity was evaluated through biochemical assays conducted on
both normal larvae and those challenged with D-galactose (D- gal).
Place and Duration of Study: Department
of Zoology, Savitribai Phule Pune University, Pune between June 2009 and July
2010.
Methodology: The M. domestica larvae were subjected to different doses of AA to
access the pro-oxidant nature. This study also investigated the antioxidant
properties of AA in the D- gal induced oxidative stress condition by assessing
the parameters as biochemical assays (Superoxide dismutase, Catalase,
Glutathione S transferase, Lipid peroxidation and Protein carbonylation) and
larval survival post-treatment with AA and D- gal.
Results: A notable association was seen
between larval mortality and AA concentration, with optimal survival at 20 mM
and complete mortality at 100 mM, due to AA's pro-oxidant properties. At a
dosage of 10 mM, AA considerably mitigated the oxidative stress generated by
D-gal (p < 0.05) and preserved both pupal survival and eclosion rates.
Conclusion: The transitional behavior of
AA in M. domestica larvae is
concentration-dependent, with reduced concentrations improving survival rates
and higher concentrations resulting in increased mortality, underscoring the
necessity for meticulous dose evaluation before administration in biological
systems.
Author (s) Details
Prajakta A. Dhage
Department of Zoology, K.R.T. Arts, B.H.
Commerce and A.M. Science College (KTHM College), Nashik-422002, India.
Archana A. Sharbidre
Department of Zoology, Savitribai Phule
Pune University, Pune 411007, MS, India.
Please see the book here:- https://doi.org/10.9734/bpi/cbrp/v1/3963
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