Background: Allergic asthma is a chronic inflammatory alteration of lung airways stimulated by immune-mediated reactions. The most represented symptoms of asthma are cough, chest tightness, shortness of breath and wheezing.
Purpose: To determine the inhibitory effect of Asparagus
officinalis extract (AOE) on allergic asthma induced by carrageenan (CGN).
Methods: Forty male mice were divided into 4 groups: the 1st group
was untreated control; the 2nd group was treated orally and daily with 500
mg/kg AOE for one week; the 3rd group was treated with a single dose of CGN 2 %
w/v (200 µL/mice) intraperitoneally and left for one week; while the 4th group
was treated first with CGN as in the 3rd group and treated with AOE as in the
2nd group after CGN injection for one week. After treatment, the animals were
sacrificed, and blood samples were subjected to white blood cell count. IL-6
and TNF-α was measured in the lung homogenate while histopathological analysis
was performed for lung samples.
Results: A single dose of CGN resulted in a significant increase
in white blood cell (WBC) count and pro-inflammatory cytokines IL-6 (490) and
TNF-α (980) pg/ml; p = 0). Histopathological analysis showed severe lung
alterations such as accumulation of infiltration cells that blocked alveolar
sacs, and over-secretion of collagenous fibers, extracellular matrix, and
hyaline membranes. Moreover, treatment with AOE after CGN injection
significantly reduced WBC count (14x109) (p = 0) and pro-inflammatory cytokines
(IL-6 (300) and TNF-α (830)pg/ml) that were raised by CGN (p = 0). Furthermore,
AOE reduced the pathological signs that were induced by the matrix, and to the
improvement of lung function and reduction of collagenous fibers and hyaline
membranes.
Conclusion: Carrageenan is an allergen-induced inflammatory agent
and causes marked pathological alterations, thereby increasing pulmonary
pathological scarring in addition to increasing the WBC count and
pro-inflammatory cytokines. Asparagus officinalis extract reduces the allergic
effect and lung pathological signs induced by CGN. Since AOE inhibits the
production of inflammatory cytokines, it has the potential to be developed as a
source of active pharmaceutical ingredients for the management of lung and
airway injury.
Author
(s) Details
Doaa M Elnagar
Zoology Department, College of Science, King Saud University, Saudi Arabia
and Zoology Department, Faculty of Women, Ain Shams University, Egypt.
Sarah A Banaeem
Zoology Department, College of Science, King Saud University, Saudi Arabia.
Wejdan S AL-Qahtani
Zoology Department, College of Science, King Saud University, Saudi Arabia.
Norah M Al- Qahtani
Zoology Department, College of Science, King Saud University, Saudi Arabia.
Ahmed M Rady
Zoology Department, College of Science, King Saud University, Saudi Arabia.
Khalid E Ibrahim
Zoology Department, College of Science, King Saud University, Saudi Arabia.
Aisha H Alqarni
Zoology Department, College of Science, King Saud University, Saudi Arabia.
Shaden A Alshareef
Zoology Department, College of Science, King Saud University, Saudi Arabia.
Samiah A Almalki
Zoology Department, College of Science, King Saud University, Saudi Arabia.
Nuha M Alabdulhadi
Zoology Department, College of Science, King Saud University, Saudi Arabia.
Please see the book here:- https://doi.org/10.9734/bpi/psnid/v3/4112
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