Gelatin Nanoparticles Containing Cantigi Leaf Extract as Anti-Agings | Chapter 5 | Pharmaceutical Science: New Insights and Developments Vol. 3

Cantigi (Vaccinium varingiaefolium Blume Miq.) has young leaves with a red color that may contain flavonoids. Flavonoids may have anti-aging activity as antioxidants, anti-collagenase, and anti-tyrosinase. The study aims to produce an ethanolic extract of Cantigi leaves and nanoparticles of the ethanolic extract and compare their antioxidant, anti-collagenase, and anti-tyrosinase activities. To make the ethanolic extract, dry powders of young Cantigi leaves are macerated gradually with hexane, ethyl acetate, and ethanol. The dried extracts are obtained after evaporating using a vacuum evaporator and then tested for specific and non-specific parameters. The dried extract is synthesized into nanoparticles by desolvation using gelatin as the polymer and glutaraldehyde as the crosslinker and then characterized. The antioxidant, anti-collagenase, and anti-tyrosinase activities of the extracts and nanoparticles are then measured and compared with their IC50s. The results show that the ethanol extract of Cantigi leaves meets specific and non-specific parameters. Moreover, the nanoparticles have a particle size of 307.90±2.54 nm, polydispersity index of 0.271±0.01, zeta potential of 12.80±0.44 mV, entrapment efficiency of 43.29±3.97%, and spherical. The IC50s of anti-collagenase, antioxidant, and anti-tyrosinase activities for extracts are 104.18±0.33, 18.87±0.36, 196.88±20.28 ppm, while for nanoparticles are 124.40±0.39, 30.14±0.33, and 53.55±27.07 ppm, respectively. The results conclude that the extract contains anti-collagenase, antioxidants, and anti-tyrosinase, and the activity is higher than the nanoparticle activity except for the anti-tyrosinase activity. The slow release of the extract from the nanoparticles may cause a decrease in activity.

 

Author (s) Details

 

Kosasih Kosasih

Fakultas Farmasi, Universitas Pancasila, Jakarta, Indonesia.

 

I Wayan Redja
Fakultas Farmasi, Universitas Pancasila, Jakarta, Indonesia.

 

Yunahara Farida
Fakultas Farmasi, Universitas Pancasila, Jakarta, Indonesia.

 

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