Hydrogen Gas Inhalation (4%) could Reduce Lung Inflammation and Blood Pressure in Rats with Monocrotaline-Induced Pulmonary Hypertension | Chapter 9 | Medicine and Medical Research: New Perspectives Vol. 2

 

The aim of this study was to investigate the effects of inhaling atmospheric air containing 4% hydrogen on the symptoms and progression of PH in male Wistar rats using the MCT-induced model. Interstitial lung diseases (IPD), associated with inflammatory processes and the appearance of fibrosis, lead to impaired ventilation-perfusion ratio and hypoxia. Recently, strategies and drug approaches for the treatment of IPD-associated pulmonary hypertension have been widely discussed. Hydrogen has been shown to exhibit selective antioxidant properties against hydroxyl radicals and exert antioxidant and anti-inflammatory effects. Because monocrotaline causes oxidative stress, the monocrotaline-induced model of pulmonary hypertension is appropriate for researching compounds having antioxidant properties. On day 1, male Wistar rats were subcutaneously injected with either a water-alcohol solution of monocrotaline or control solution consisting solely of water and alcohol. One group of monocrotaline-injected animals was placed in a plastic box continuously ventilated with atmospheric air containing 4% molecular hydrogen, while the two other groups (one injected with monocrotaline and the other with vehicle) were placed in boxes ventilated with atmospheric air. After 21 days, hemodynamic parameters were measured under urethane narcosis. The results showed that, although hydrogen inhalation had no effect on the main markers of pulmonary hypertension induced by monocrotaline injection, there was a reduction in systemic blood pressure due to its systolic component, and a decrease in TGF- β expression, as well as a reduction in tryptase-containing mast cells. The results in combination with previous published results indicate that molecular hydrogen may be a useful therapeutic agent to combat pulmonary hypertension. Further research with larger sample sizes and exploring different routes of administration, concentrations and durations is needed to determine the optimal therapeutic approach. Overall, these findings suggest that molecular hydrogen may hold promise as a therapeutic agent in the treatment of pulmonary hypertension, in patients but further investigation is warranted.

 

Author(s) Details

Tatyana Kuropatkina
Faculty of Medicine, Lomonosov Moscow State University, Lomonosovsky Prospect 27-1, 119991 Moscow, Russia.

 

Dmitrii Atiakshin
Research Institute of Experimental Biology and Medicine, N.N. Burdenko Voronezh State Medical University, Moskovsky Prispect, 185, 394066, Voronezh, Russia and RUDN University, Miklukho-Maklaya St 6, 117198, Moscow, Russia.

 

 

Fedor Sychev
Faculty of Biology, Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia.

 

Marina Artemieva
Faculty of Medicine, Lomonosov Moscow State University, Lomonosovsky Prospect 27-1, 119991 Moscow, Russia and Faculty of Biology, Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia.

 

Tatyana Samoilenko
Research Institute of Experimental Biology and Medicine, N.N. Burdenko Voronezh State Medical University, Moskovsky Prispect, 185, 394066, Voronezh, Russia.

 

Olga Gerasimova
Research Institute of Experimental Biology and Medicine, N.N. Burdenko Voronezh State Medical University, Moskovsky Prispect, 185, 394066, Voronezh, Russia.

 

Viktoria Shishkina
Research Institute of Experimental Biology and Medicine, N.N. Burdenko Voronezh State Medical University, Moskovsky Prispect, 185, 394066, Voronezh, Russia.

 

Khaydar Gufranov
Faculty of Medicine, Lomonosov Moscow State University, Lomonosovsky Prospect 27-1, 119991 Moscow, Russia.

 

Natalia Medvedeva
Faculty of Biology, Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia.

 

Tyler W. Le Baron
Department of Kinesiology and Outdoor Recreation, Southern Utah University, Cedar City, 84720, Utah, USA and Molecular Hydrogen Institute, Cedar City, Utah, 84720, USA.

 

Oleg Medvedev
Faculty of Medicine, Lomonosov Moscow State University, Lomonosovsky Prospect 27-1, 119991 Moscow, Russia and Laboratory of Experimental Pharmacology, National Medical Research Center of Cardiology, Ulitsa Akademika Chazova 15a, 121552 Moscow, Russia.

 

 

Please see the book here:- https://doi.org/10.9734/bpi/mmrnp/v2/1180

Beneficial Effects of Hydrogen Water on Subjects with NAFLD | Chapter 8 | Research Developments in Medicine and Medical Science Vol. 4

 Non-alcoholic greasy liver disease (NAFLD) is a liver disorder that influences approximately a quarter of the all-encompassing population. It is caused by an exuberance of oxidative stress, inflammation, and irregular cellular indicating, which can influence liver dysfunction and eventually, carcinoma. Molecular hydrogen has existed identified for allure specific antioxidant characteristics and ability to decrease inflammation and regulate natural activity. In a randomized, double-blind, fake pill-controlled trial unending eight weeks, hydrogen-rich water (HRW) was given to 30 matters with NAFLD. We noticed approving trends (p > 0.05) in abated weight (about 1 kg) and party mass index in the HRW group. HRW was well-indulged, with no significant changes in liver enzymes and an slant towards improved lipid sketch and lower lactate dehydrogenase levels. HRW went to decrease nuclear determinant kappa B, heat shock protein 70, and matrix metalloproteinase-9 levels non-significantly. Curiously, skilled was a slight but non-significant increase in the levels of 8-hydroxy-2’-deoxyguanosine and malondialdehyde in the HRW group. This gentle increase may desire hormetic effects of microscopic hydrogen. Further, longer-term studies focused on barring, maintaining, or discussing NAFLD and early stages of NASH are necessary.

Author(s) Details:

Branislav Kura

Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, 841 04 Bratislava, Slovak Republic.

 

Maria Szantova

3rd Department of Internal Medicine, Faculty of Medicine, Comenius University, 813 72 Bratislava, Slovak Republic.

 

Tyler W. LeBaron

Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, 841 04 Bratislava, Slovak Republic, Molecular Hydrogen Institute, 847 21 Enoch, UT, USA and Department of Kinesiology and Outdoor Recreation, Southern Utah University, 847 21Cedar City, UT, USA.

 

Viliam Mojto

3rd Department of Internal Medicine, Faculty of Medicine, Comenius University, 813 72 Bratislava, Slovak Republic.

 

Miroslav Barancik

Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, 841 04 Bratislava, Slovak Republic.

 

Barbara Szeiffova Bacova

Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, 841 04 Bratislava, Slovak Republic.

 

Barbora Kalocayova

Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, 841 04 Bratislava, Slovak Republic.

 

Matus Sykora

Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, 841 04 Bratislava, Slovak Republic.

 

Ludmila Okruhlicova

Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, 841 04 Bratislava, Slovak Republic.

 

Narcisa Tribulova

Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, 841 04 Bratislava, Slovak Republic.

 

Anna Gvozdjakova

Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University Bratislava, 811 08 Bratislava, Slovak Republic.

 

Zuzana Sumbalova

Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University Bratislava, 811 08 Bratislava, Slovak Republic.

 

Jarmila Kucharska

Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University Bratislava, 811 08 Bratislava, Slovak Republic.

 

Xenia Faktorova

Internal Clinic of Slovak Medical University, Hospital of St. Michael, 811 08 Bratislava, Slovak Republic.

 

Martina Jakabovicova

3rd Department of Internal Medicine, Faculty of Medicine, Comenius University, 813 72 Bratislava, Slovak Republic. 

Zuzana Durkovicová

3rd Department of Internal Medicine, Faculty of Medicine, Comenius University, 813 72 Bratislava, Slovak Republic. 

Jan Macutek

Mathematical Institute, Slovak Academy of Sciences 814 73 Bratislava, Slovak Republic and Department of Mathematics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, 949 01 Nitra, Slovak Republic.

 

Michaela Koscová

Mathematical Institute, Slovak Academy of Sciences 814 73 Bratislava, Slovak Republic.

 

Jan Slezak

Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, 841 04 Bratislava, Slovak Republic.

Please see the link here: https://stm.bookpi.org/RDMMS-V4/article/view/9837

An Overview on Male Infertility and Stress Factors | Chapter 11 | Perspective of Recent Advances in Medical Research Vol. 9

 Infertility is a friendly issue that adds to the troubles of marital life. Sex is ultimate powerful idea and drive in all living beings, containing humans. Infertility can be created by a decrease in the number of sperms, the absence of sperms, or an increase in the uncommon form of sperms in male sperm, as well as the semen's inability to move accompanying sufficient vigour towards the female reproductive cell. Infertility is caused by reactive oxygen class (ROS). Depending on the nature and aggregation of the ROS deeply operating, these ROS can have both beneficial and disadvantageous effects on semen functions. The hormones initially judged for infertility in males contain follicle exciting hormone (FSH), luteinizing hormone (LH), testosterone and prolactin. Hydrogen whiten (H2O2) is a major poisonous ROS for human spermatozoa, causing semen immobilisation at low concentrations. H2O2 at high concentrations causes lipid peroxidation, that leads to cell cessation. The superoxide anion (O2-) on the other hand performs to have a significant role while sperm learning disability and capacitation.

Author(s) Details:

Anupama Sharma,
Department of Biochemistry, College of Paramedical Technology, Nims University, Jaipur, Rajasthan, India.

Girish Chandra Sharma,
Department of Chemistry, Jaypee University Anoopshahr, India.

Please see the link here: https://stm.bookpi.org/PRAMR-V9/article/view/9609

Use of Micronutrients for Treatment of Idiopathic Abnormal Sperm Parameters| Chapter 7 | Current Overview on Disease and Health Research Vol. 9

 The presnt study aimed to look at the possible effects of micronutrients on semen parameters including sperm count, motility, and morphology to improve male infertility. WHO defines infertility as the inability to achieve pregnancy within 12 months of regular sexual intercourse for couples in conception. Infertility affects 13–20% of couples in Poland and around the world, regardless of race or ethnicity. This study started from the 1st of March 2016 until 30th of November 2016 (6 six months was the period of taking the treatment) while the duration time of the study including the follow up and the examination period was 9 nine months.

In this clinical trial included male subjects aged from 20 to 60 years with at least 1 year of infertility and abnormal semen analysis who received 3 -6 months’ treatment with a Cyclofert men that contains (l-carnitine l-tartarate 1g, N – Asetyl - cysteine 600 mg, 40 mg zinc, Lycopene 20 mg, 60 mg vitamin E, 200 mg vitamin C, 80 mg glutathione, 200 g selenium, 100 mg coenzyme Q10 and 400 g folic acid. Theses micronutrients were given in a form of two tablets/ twice a day for 6 months). Sperm parameters (count, motility, and morphology) and pregnancy incidence were monitored before and after treatment. After three and six months of treatment, the data gathered indicated that there was an overall progressive motility. Furthermore, compared to the untreated group, the sperm count rose by 64% after three months and 88% after six months ( = 0.00, 1, at P 0.05). Additionally, treatment for three and six months markedly improved the morphology of the sperm (p 0.05). Following six months of treatment, 30% of women were able to become pregnant thanks to these beneficial changes. However, the length of treatment has a direct correlation with each of these improvements. The pregnancy incidence has been increased compared to the untreated group. This treatment improved also the pregnancy status in which it caused an elevation in the number of pregnant spouses compared with untreated ones.

Toxicity Preventive Potential of Anti-Inflammatory CeO2 Nanoparticles in HUVE Cells | Chapter 8 | Recent Trends in Chemical and Material Sciences Vol.7

The mechanism behind cerium oxide nanoparticles' (CeO2 NPs) cytoprotective capability against cytotoxic nitric oxide (NO) donors and H2O2 is currently unknown. The lipopolysaccharide (LPS)-induced cytokines IL-1 and TNF- were dramatically reduced by CeO2 NPs that had been synthesised and described. Because NP-induced ROS/NO did not cause toxicity in HUVE cells, the main goal of this investigation was to determine the capacities of CeO2 NPs in terms of signalling effects that could have happened owing to reactive oxygen species (ROS) and/or NO. Two NO-donors were used in conjunction with CeO2 NPs in human umbilical vein-derived endothelial (HUVE) cells to achieve this goal. To our knowledge, there has been little investigation on CeO2 NPs' modulatory ability against known NO-donors. SNP (sodium nitroprusside, #ab145732 from Abcam plc, Cambridge, UK) and DETA-NO (Z)-1-[N-(2-Aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate or DETA-NONOate #ab144627 from Abcam plc, Cambridge, UK) were the two NO donors utilised in this The IC50s of two NO donors (DETA-NO; 1250 110 M and sodium nitroprusside (SNP; 950 89 M) as well as the IC50 of H2O2 (120 7 M) were used to assess cytoprotective capacity and its underlying mechanism. Total ROS (as a collective marker of hydrogen peroxide, superoxide radical, hydroxyl radical, and other ROS) was assessed using DCFH-DA, and significant ROS was deciphered using a specialised probe DHE. The data demonstrated that signalling processes mediated primarily by and/or NO are responsible for CeO2 NPs at 100 g/mL ameliorating toxicity. The fact that NP exposure had no effect on mitochondrial membrane potential (MMP) and that CeO2 NPs-mediated recovery of MMP loss caused by exogenous NO donors and H2O2 suggested that NP-mediated generation was extra-mitochondrial. The mechanism behind the NP-induced gain in GSH and persistent cytoplasmic ROS is partially explained by data on activated glutathione reductase (GR) and unchanged glutathione peroxidase (GPx) activity. Instead of being inhibited by CeO2 NP administration, cells may develop the ability to tolerate exogenously generated toxicity due to increased antioxidant capacity due to non-cytotoxic ROS and/or NO generation.

Author(s) Details:

Mohd Javed Akhtar,
King Abdullah Institute for Nanotechnology, King Saud University, Riyadh-11451, Saudi Arabia.


Maqusood Ahamed,
King Abdullah Institute for Nanotechnology, King Saud University, Riyadh-11451, Saudi Arabia.

Please see the link here: https://stm.bookpi.org/RTCAMS-V7/article/view/6101

Investigating the Effects of Oxidative Stress Prevention Using DNA Aptamer (Aptamin C®) in Keratinocyte | Chapter 2 | Highlights on Medicine and Medical Science Vol. 17

 One of the primary causes of many diseases is oxidative stress. Reactive oxygen species (ROS) levels exceed the cell's defence mechanisms, resulting in oxidative stress. Antioxidants are efficient against reactive oxygen species (ROS), which are a significant cause of oxidative stress. Antioxidants are crucial in our bodies because they scavenge free radicals, preventing them from causing damage to biological molecules. Vitamin C is a key antioxidant and is required for a variety of physiological processes such as collagen formation and neurotransmitter synthesis. Although vitamin C is widely utilised in cosmetic and medicinal products, it is easily oxidised by air, pH, temperature, and UV light, lowering its antioxidant efficacy and shortening the shelf life of vitamin C-containing products. To address this shortcoming, we created Aptamin C®, a novel single-strand DNA aptamer that boosts vitamin C's antioxidant efficiency by attaching to its reduced form and delaying oxidation. We used both in vitro and clinical testing to look into the effects of Aptamin C® and vitamin C complex on human skin. The Aptamin C® and vitamin C complex demonstrated effective ROS scavenging and anti-inflammatory actions, according to our findings.

Author (S) Details

Sooho Choi
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

Yoonjeong Hwang
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

Taejun Kim
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

Jeong Hoon Kim
Nexmos, Inc., 2207 U-Tower, 767, Sinsu-ro, Suji-gu, Yongin-si, Gyeonggi-do, Republic of Korea.

View Book :- https://stm.bookpi.org/HMMS-V17/article/view/2605

Modulation of Apoptotic Cell Death and Neuroprotective Effects of Glutathione—L-Dopa Codrug against H2O2-Induced Cellular Toxicity: A Recent Study | Chapter 11 | Current Advances in Chemistry and Biochemistry Vol. 9

 Parkinson's disease (PD) is a neurodegenerative condition characterised by the death of dopaminergic neurons, primarily in the substantia nigra, over time. The gold standard medicine for managing Parkinson's disease (PD) and controlling its symptoms is L-3,4-dihydroxyphenylalanine (LD). However, the formation of pro-oxidant intermediates resulting from LD's autoxidation could produce disease neurotoxicity. To circumvent this constraint, we created a codrug by conjugating LD to the natural antioxidant glutathione (GSH) (GSH-LD). The effect of GSH-LD on H2O2-induced cellular toxicity was investigated in the undifferentiated and differentiated lymphoma U-937 and dopaminergic neuroblastoma SH-SY5Y cell lines, which were used as models to study the involvement of macrophages/microglia and dopaminergic neurons in PD, respectively. We looked studied how GSH-LD affected apoptosis and cellular oxidative stress, two important targets in the prevention and therapy of neurodegenerative disorders. In both cell lines, GSH-LD showed a larger effect than LD and GSH in preventing hydrogen peroxide (H2O2)-induced apoptosis. In a neurotoxicity cellular model, GSH-LD was also able to protect cell viability, cellular redox state, gluthation metabolism, and inhibit reactive oxygen species (ROS) generation in a PI3K/kinase B (Akt)-dependent manner. Our findings suggest that the GSH-LD codrug has benefits derived from the synergistic impact of LD and GSH, and that it could be a good PD treatment candidate. Further research into the neuroprotective benefits of GSH-LD in PD in in vivo animal models and its capacity to permeate the BBB is needed to confirm the importance of our findings.

Author (s) Details

Sara Franceschelli
Department of Psychological, Health and Territorial Sciences, University “G. D’Annunzio”, 66100 Chieti-Pescara, Italy.

Paola Lanuti
Department of Medicine and Science of Aging, University “G. D’Annunzio”, 66100 Chieti-Pescara, Italy.

Alessio Ferrone
Department of Medicine and Science of Aging, University “G. D’Annunzio”, 66100 Chieti-Pescara, Italy.

Daniela Maria Pia Gatta
Department of Medicine and Science of Aging, University “G. D’Annunzio”, 66100 Chieti-Pescara, Italy.

Lorenza Speranza
Department of Medicine and Science of Aging, University “G. D’Annunzio”, 66100 Chieti-Pescara, Italy.

Mirko Pesce
Department of Medicine and Science of Aging, University “G. D’Annunzio”, 66100 Chieti-Pescara, Italy.

Alfredo Grilli
Department of Psychological, Health and Territorial Sciences, University “G. D’Annunzio”, 66100 Chieti-Pescara, Italy.

Ivana Cacciatore
Department of Pharmacy, University “Gabriele D’Annunzio” of Chieti-Pescara, 66100 Chieti-Pescara, Italy.

Emanuela Ricciotti
Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, Philadelphia, PA 19104, USA.

Antonio Di Stefano
Department of Pharmacy, University “Gabriele D’Annunzio” of Chieti-Pescara, 66100 Chieti-Pescara, Italy.

Sebastiano Miscia
Department of Medicine and Science of Aging, University “G. D’Annunzio”, 66100 Chieti-Pescara, Italy.

Mario Felaco
Department of Medicine and Science of Aging, University “G. D’Annunzio”, 66100 Chieti-Pescara, Italy.

Antonia Patruno
Department of Medicine and Science of Aging, University “G. D’Annunzio”, 66100 Chieti-Pescara, Italy.

View Book :- https://stm.bookpi.org/CACB-V9/article/view/2202