Antimicrobial resistance is a matter of great concern due to the
reduced efficacy or failures of antibiotics to treat various bacterial
infections. At present, there are few reports available on the isolation and
prevalence of antibiotic resistance genes on E. coli found in wild captive
animal species. Therefore, the present study was conducted to investigate the
phenotypic and genotypic properties of E. coli isolates obtained from wild
captive animals at Bikaner Zoo in regard to their antibiotic resistance. In the
present study, a total of 41 E. coli isolates obtained from Boselaphus
tragocamelus (5), Antelope cervicapra (18) and Gazella gazelle (18) maintained
at Bikaner Zoo (Rajasthan) were subjected to antibiogram determination against
15 antibiotics belonging to four different classes and were also genotyped for
detecting presence of blaTEM, sul-2, strA and aadA genes. Antimicrobial
susceptibility was performed by the agar disc diffusion method. The polymerase
chain reaction was carried out with a Veriti 96 well thermocycler to detect the
presence of the identified genes amplified through specific primers.
Antibiogram study revealed the highest efficacy of ciprofloxacin (90.2%)
followed by nalidixic acid (75.6%) and chloramphenicol (68.4%) and high resistance
to \(\beta\) lactam, Sulfamethoxazole and Aminoglycoside antibiotics. The
overall presence of blaTEM, sul-2, strA and aadA genes was detected in 95.12%,
80.48%, 60.97% and 87.8% in isolates. This study's findings reveal that wild
captive animals may act as a reservoir of antibiotic resistant genes which may
be mobilized into other ecological populations. The data from the present study
accounts for the critical need for regulation of antimicrobial drug usage in
wild captive animals and highlights for continuous monitoring of antibiotic
resistance.
Author(s)
Details
Sophia
Zaidi
Government College, Deshnok-334801, Bikaner, Rajasthan, India.
Please see the link:- https://doi.org/10.9734/bpi/rpmab/v5/349
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