Characterisation of Canine-derived Lactic Acid Bacteria from Faecal and Oral Sources: Potential for Probiotic Use in Dog Health| Chapter 7 | Microbiology and Biotechnology Research: An Overview Vol. 3

 

Probiotic bacteria are live microorganisms primarily used to control gut bacterial, viral, and parasitic infections. The gut microflora plays a crucial role in maintaining the overall health of animals and supporting normal digestive functions. While probiotics have traditionally been used in human nutrition, their application has now extended to animals. Lactic acid bacteria constitute a major group of probiotic bacteria to be used as a probiotic supplement for animals. The lactic acid bacteria are always species specific, but none of the commercial probiotic products for dogs are of canine origin. Due to the limited probiotic research in dogs, the aim of the study is to isolate and characterise the lactic acid bacteria from canine faecal samples to develop species-specific probiotic supplements tailored for dogs. A total 160 numbers of Canine oral and faecal lactic acid bacteria Strains were isolated from 60 healthy dogs. Lactic Acid Bacteria were found in 37% of canine faecal samples and 9 % of canine oral samples. Then these samples were cultivated on lactobacillus selective media with acetic acid. The probiotic strains were analysed based on the frequency and quantity in faeces, growth density, acid tolerance, antimicrobial activity, biochemical test and antibiotic sensitivity test. Among 160 fresh oral and faecal samples, 60 faecal samples and 15 oral samples were shown to be positive for lactic acid bacteria grown in lactobacillus selective media, MRS broth and Rogosa agar. The broad-spectrum group of antibiotics is used to check the antibiotic susceptibility, and a few commonly used antibiotics have maximum zone of inhibition in the probiotic culture. The isolates show positive for the catalase test, nitrate reduction test, and citrate test. The lactic acid bacteria strain present in oral and faecal samples was identified by partial 16s rRNA gene sequencing of a fragment amplified by PCR using universal primers. A 900bp PCR product was obtained in a 1% agarose gel. Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus casei, Lactobacillus mucosae, Lactobacillus delbrueckii and Lactobacillus salivarius were most frequently encountered bacteria in the canine oral cavity and faeces. Sequence similarity values of 93 % with Lactobacillus casei, 89% with Lactobacillus pentosus, and 85% with Lactobacillus delbrueckii were found. The findings highlight the need for a novel probiotic strain of canine origin in India. Keeping this point, the present study was carried out with the objective of detecting of suitable Lactobacillus spp. from dog faeces for usage as probiotic in future and the in vitro antibacterial activity results highlighted and further in-vivo studies are needed in India to establish potential probiotic bacteria of canine faecal Lactobacillus species in the future.

 

Author (s) Details

G. Kalaiselvi
Central University Laboratory, Centre for Animal Health Studies, Chennai -51, India.

N. Pazhanivel
Central University Laboratory, Centre for Animal Health Studies, Chennai -51, India.

 

Sumedha S. Bobade
Madras Veterinary College, TANUVAS, Chennai-51, India.

 

Please see the book here:- https://doi.org/10.9734/bpi/mbrao/v3/5580