Aim: To prove the antibacterial sensitivity of phytogenic silver nanoparticles against food spoilage causing organisms such as Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923).
Study Design: Extract based
on raw banana peels harnessed from chips manufacturing units was utilized as
the reducing agent for the biosynthesis of silver nanoparticles, followed by
evaluation of their antibacterial efficacy against common food spoilage
organisms through agar diffusion analysis. The experiments were conducted in
triplicate, and the average values were depicted graphically.
Place and Duration of Study: Sahrdaya College of Engineering and
Technology, Kodakara and Centre for Research on Molecular and Applied Sciences,
Thiruvananthapuram, between June to August 2023.
Methodology: Raw banana peel extract was utilized as plant based
reducing agent in bioinspired synthesis of silver nanoparticles. The
bio-inspired silver nanoparticles formation was confirmed using surface plasmon
resonance studies. The biosynthesized particles were isolated through
centrifugation and subjected to characterization through Scanning Electron
Microscopy (SEM) and Energy Dispersive X-ray (EDX) analysis to assess their
physical structure and chemical composition. The antibacterial efficacy of
these particles was assessed through agar diffusion assay, and the minimum
concentration of nanoparticles solution to inhibit the microbial growth was
determined against food spoilage organisms.
Results: Spherical shaped silver nanoparticles with in the size
range of 56-80 nm was synthesized using raw banana peel as reducing agent.
These nanoparticles exhibited minimum inhibitory concentrations of 56.07 µg/mL
and 79.32 µg/mL, as well as minimum bactericidal concentrations of 156.59 µg/mL
and 188.96 µg/mL against Escherichia coli
and Staphylococcus aureus
respectively.
Conclusion: The silver nanoparticles synthesized with raw banana
peel extract as the reducing agent demonstrated antibacterial sensitivity
against food spoilage organisms.
Author
(s) Details
Sumi
Maria Babu
Department of Biotechnology, Sahrdaya College of Engineering and
Technology, Kodakara, Affiliated to APJ Abdul Kalam Technological University,
Thiruvananthapuram, India.
Leon
Ittiachen
Department of Biotechnology, Sahrdaya College of Engineering and
Technology, Kodakara, Affiliated to APJ Abdul Kalam Technological University,
Thiruvananthapuram, India.
Please see the book here:-
https://doi.org/10.9734/bpi/mono/978-93-49238-47-3/CH35
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