Tuesday, 23 December 2025

High-Performance Liquid Chromatographic Method Development and Validation for Cefdinir Assessment Using Tinidazole as an Internal Standard in Bulk and Pharmaceutical Dosage Forms | Chapter 8 | Chemistry and Biochemistry: Research Progress Vol. 8

 

Cefdinir is a semi-synthetic oral antibiotic belonging to the cephalosporin family of antibiotics. For the estimation of the drug Cefdinir, several methods are available in the literature. The aim of this study was to develop a faster method for eluting Cefdinir. The present work involves a rapid and reproducible HPLC method, which was developed and comprehensively validated for the estimation of the drug cefdinir in the bulk drug and pharmaceutical dosage forms. Tinidazole was used as an internal standard. The method addresses challenges such as long run times, unstable retention behaviour, and inadequate resolution. Chromatographic separation was achieved using a C18 column under isocratic conditions with a mobile phase tailored for optimal peak shape and robustness. Validation followed ICH Q2(R1) guidelines, demonstrating excellent linearity (r² ≥ 0.999), precision (%RSD ≤ 2%), and accuracy (98–102% recovery). The method further exhibited strong ruggedness and robustness under deliberate variations. These results confirm the suitability of the developed method for routine quality-control applications. The validated method was successfully applied to the commercially available pharmaceutical dosage forms.

 

Author(s) Details

Gadapa Nirupa
Department of Chemistry, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, A.P 500085, India.

 

Upendra M. Tripathi
R and D Department, Startech Labs Pvt. Ltd., 2nd Floor, S M R Chambers, H. No 1-58/7 Madinaguda, Hyderabad, A.P, 500050, India.

 

Please see the book here :- https://doi.org/10.9734/bpi/cbrp/v8/6752

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