High-Performance Liquid Chromatographic Method Development and Validation for Cefdinir Assessment Using Tinidazole as an Internal Standard in Bulk and Pharmaceutical Dosage Forms | Chapter 8 | Chemistry and Biochemistry: Research Progress Vol. 8

 

Cefdinir is a semi-synthetic oral antibiotic belonging to the cephalosporin family of antibiotics. For the estimation of the drug Cefdinir, several methods are available in the literature. The aim of this study was to develop a faster method for eluting Cefdinir. The present work involves a rapid and reproducible HPLC method, which was developed and comprehensively validated for the estimation of the drug cefdinir in the bulk drug and pharmaceutical dosage forms. Tinidazole was used as an internal standard. The method addresses challenges such as long run times, unstable retention behaviour, and inadequate resolution. Chromatographic separation was achieved using a C18 column under isocratic conditions with a mobile phase tailored for optimal peak shape and robustness. Validation followed ICH Q2(R1) guidelines, demonstrating excellent linearity (r² ≥ 0.999), precision (%RSD ≤ 2%), and accuracy (98–102% recovery). The method further exhibited strong ruggedness and robustness under deliberate variations. These results confirm the suitability of the developed method for routine quality-control applications. The validated method was successfully applied to the commercially available pharmaceutical dosage forms.

 

Author(s) Details

Gadapa Nirupa
Department of Chemistry, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, A.P 500085, India.

 

Upendra M. Tripathi
R and D Department, Startech Labs Pvt. Ltd., 2nd Floor, S M R Chambers, H. No 1-58/7 Madinaguda, Hyderabad, A.P, 500050, India.

 

Please see the book here :- https://doi.org/10.9734/bpi/cbrp/v8/6752

Simultaneous Determination of Ofloxacin and Tinidazole from Pharmaceutical Dosage Formulation by Two Spectrophotometric Methods | Chapter 5 | Current Advances in Chemistry and Biochemistry Vol. 4

 For the estimation of Ofloxacin and Tinidazole from combined tablet dosage form, two quick, sensitive, reliable, precise, rapid, and cost-effective methods were developed. The first method uses a simultaneous equation, while the second method uses Qanalysis (absorbance ratio method). In 0. 1N MethanolicHCI (20:80), ofloxacin and tinidazole have maximum absorbances of 300 nm and 275 nm, respectively. With a Regression Coefficient (R2) greater than 0.999, linearity was achieved in the concentration ranges of 1-20 g/ml for Ofloxacin and 3-60 g/ml for Tinidazole. The first method used simultaneous equations to calculate the concentration and amount of drug, while the second method used the ratio of absorbance at the isoabsorptive point (which was found to be 286 nm) and at the maximum concentration of one of the drugs to determine the concentration and amount of drug. The findings of the study have been statistically confirmed, followed by assay and recovery tests.

Author (s) Details

Pralhad Rege
Department of Chemistry, St. Xavier’s College, Mumbai, India.

Vaibhav Salvi
Department of Chemistry, Ruia College, Mumbai, India.

Abhishek Tiwari
Department of Chemistry, St. Xavier’s College, Mumbai, India.

View Book :- https://stm.bookpi.org/CACB-V4/article/view/905