Hepatitis delta virus (HDV) was discovered in 1977 by Italian
gastroenterologist Mario Rizzetto. The small and large delta antigens (S-HDAg
and L-HDAg, respectively) represent two forms of the only protein encoded by
the hepatitis Delta virus (HDV) RNA genome. Consequently, HDV relies, to a
large extent, on the host cell machinery for replication and transcription.
Using different approaches few cellular proteins were identified as S- HDAg or
L-HDAg partners with limited evidence of their involvement in the modulation of
the virus life cycle and mechanisms of pathogenesis. Here we performed a yeast
two-hybrid screening of a human liver cDNA library to identify cellular
proteins that bind to S-HDAg. We were able to identify HuR, a ubiquitously
expressed protein involved in the regulation of gene expression at the
posttranscriptional level, and associated with cancer, as an S-HDAg partner
both in vitro and in vivo. HuR was found to be overexpressed and colocalized
with HDAg in human hepatoma cells. siRNA knockdown of HuR mRNA resulted in
inhibition of S-HDAg and L-HDAg expression. The obtained results suggest that HuR
may play an important role in the HDV replication cycle and pathogenesis.
Author(s)
Details
Ana
Casaca
Unidade de Microbiologia Médica, Global Health and Tropical
Medicine (GHTM), Associate Laboratory in Translation and Innovation Towards
Global Health, LA-REAL Instituto de Higiene e Medicina Tropical (IHMT),
Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
Celso
Cunha
Unidade de Microbiologia Médica, Global Health and Tropical
Medicine (GHTM), Associate Laboratory in
Translation and Innovation Towards Global Health, LA-REAL Instituto de Higiene
e Medicina Tropical (IHMT), Universidade Nova de Lisboa, Rua da Junqueira 100,
1349-008 Lisboa, Portugal.
Please see the link:- https://doi.org/10.9734/bpi/rpmab/v5/1258
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