Monday, 17 February 2025

Examining and Calculating the pH of 2-[N-Morpholino]ethanesulfonic Acid (MES) Buffer Solution from 5˚C to 55˚C | Chapter 1 | Chemical and Materials Sciences: Developments and Innovations Vol. 10

In biomedical, biological, and clinical laboratories, knowledge of the pH of blood and physiological fluids is of great importance. This paper reports the results for the pH of three buffer solutions free of chloride ions. The remaining six buffer solutions have saline media of ionic strength I=0.16 mol kg-1 matching closely to that of the physiological sample. Conventional paH values for the three buffer solutions without the chloride ion and six buffer solutions with the chloride ion at I=0.16 mol kg-1 from 5 C to 55 C have been calculated. The operational pH values for five buffer solutions at 5 C to 55C have been determined based on the difference in the values of the liquid junction potentials between the blood phosphate standard and the experimental buffer solutions. Five of these buffers are recommended as standards for the physiological pH range of 7.5 to 8.5. It was concluded that all emf data are stable, reliable, and accurate. The MES buffer solutions are considered standards of assigned pH and will be useful when buffer solutions of known conventional pH are required.

 

Author (s) Details

Lakshmi N. Roy
Hoffman Department Of Chemistry, Drury University, Springfield, Usa.

 

Rabindra N. Roy
Hoffman Department Of Chemistry, Drury University, Springfield, Usa.

 

Joshua T. Wollen
Hoffman Department Of Chemistry, Drury University, Springfield, Usa.

 

Jessica M. Stegner
Hoffman Department Of Chemistry, Drury University, Springfield, Usa.

 

Meagan A. Harmon
Hoffman Department Of Chemistry, Drury University, Springfield, Usa.

 

Michael S. Martin
Hoffman Department Of Chemistry, Drury University, Springfield, Usa.

 

Blake M. Bodendorfer
Hoffman Department Of Chemistry, Drury University, Springfield, Usa.

 

Isaac B. Henson
Hoffman Department Of Chemistry, Drury University, Springfield, Usa.

 

Please See The Book Here:- Https://Doi.Org/10.9734/Bpi/Cmsdi/V10/3473

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