Non-Saccharomyces
yeast strains eat a wide variety of carbohydrates and can produce ethanol in
varying amounts and concentrations. In Nigeria, wild type indigenous strains of
Saccharomyces cerevisae that can compete with commercial strains of
Saccharomyces cerevisae are uncommon. The goal of this study was to compare
Meyerozyma guilliermondii's ability to ingest sugars (fructose, galactose,
glucose, lactose, sucrose, and molasses) and convert them to ethanol during
fermentation with a strain of Saccharomyces cerevisiae. Different quantities of
fructose (5g/L, 10g/L, 20g/L, 30g/L) were added to broth containing yeast
extract (6g/L), peptone (10g/L), and malt extract (6g/L). galactose, glucose,
lactose, and sucrose are the sugars galactose, glucose, lactose, and sucrose,
respectively. A refractometer was used to quantify sugar usage after 96 hours
of incubation at 120 rpm and 30oC. Meyerozyma guilliermondii had a considerably
greater alcoholic production utilising molasses (9.20.45 mg/ml) than
Saccharomyces cerevisiae strain T (4.81.15 mg/ml) at 96 hours. For M.
guilliermondii, ethanol production from fructose as the sole carbon source was
2.1, 3.0, 8.11, and 9.06 (mg/ml) compared to 1.08, 3.12, 8.06, and 6.0 (mg/ml)
for S. cerevisiae. At all doses tested, both strains showed equivalent
adaptability to galactose metabolism. At 1.0 percent (4.15, 3.18 mg/ml) and 2.0
percent glucose (4.25, 3.3 mg/ml), M. guilliermondii produced more glucose than
S. cerevisiae. M. guilliermondii and S. cerevisiae produced 8.15 and 9.08 mg/ml
ethanol, respectively, at 3.0% glucose broth concentration. At 3.0% sugar
supplement, M. guilliermondi and S. cerevisiae yielded 10.18 mg/ml ethanol,
compared to 7.06 mg/ml for M. guilliermondi and S. cerevisiae, respectively.
Meyerozyma guilliermondii shown its versatility as a non-Saccharomyces yeast
specie capable of making ethanol from a wide range of sugars found in local
feedstock as a viable alternative.
Author (S) Details
Gidado Rose Suniso Maxwell
Department of
Agricultural Biotechnology, National Biotechnology Development Agency (NABDA),
Abuja, Nigeria.
Isah Abraham
Department of Biochemistry, Ahmadu Bello University Zaria, North West,
Nigeria.
Iweajunwa Sarah
Department of Agricultural Biotechnology, National Biotechnology
Development Agency (NABDA), Abuja, Nigeria.
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Book :- https://stm.bookpi.org/NVBS-V1/article/view/3174