Aim: The present study investigated the effects of metformin (MET) in protection against oxidative stress and pancreatic damage using an animal model, to assess the effect of metformin on oxidative stress in vivo and in vitro.
Introduction: Diabetes mellitus is a worldwide health
problem that has a deleterious impact on life expectancy and quality. People
with diabetes have a higher oxidation state, which is detrimental to
homeostasis. Numerous tissues have demonstrated the anti-oxidant qualities of
metformin. An oral medication for diabetes called metformin aids in blood sugar
regulation.
Methods: This study investigated the effect of metformin on
total antioxidant markers in the serum and pancreatic tissue of Alloxan-induced
diabetic rats. Diabetic rats were randomised into five groups for treatment and
received metformin (100,200,300 mg/kg) orally once daily for four weeks. The
antioxidant potential of evaluated total antioxidant status (TAOS) in vivo and
through DPPH (1,1-diphenyl-2-picryl-hydrazyl) scavenging assay at 517 nm in
vitro.
Results: A Considerable drop in elevated blood glucose level
was observed in the alloxan-induced diabetic. At a dose of 100,200,300mg/kg
demonstrated increase significantly improvement rate percentage in (M100, M200,
M300) were (40.701 %),(48.063%) and (48.101%) respectively after end of
treatment compared to diabetic group that decrease (-243.340%), TAOS were
significantly increased in treated group than diabetic group" p= 0.000*
".The percentage of improvement rate elevation in treatment M100, M200,
and M300 was (133.202 %,148.433% and 194.358%) respectively, compared with
diabetic, while that percentage was decreased (-65.677%). Also, it improved
pancreas histopathology compared to the diabetic control group it improved pancreas
histopathology compared to the diabetic control group Antioxidant activity
using DPPH was found to increase in a concentration-time-dependent manner IC50
of metformin (498.0 µg/ml), while ascorbic acid IC50 (µg/ml 29.62).
Conclusions: In "alloxan-induced diabetic rats",
metformin provides "protective actions" against free radicals and
oxidative stress, as well as improving pancreas tissue histology and lowering
blood glucose levels.
Author (s) Details
Abeer Mansour Abdel
Rasool
Department of Pharmacology, Collage of Pharmacy, University of Nineveh,
Iraq.
Isam Hamo Mahmood
Department of Pharmacology, AL Noor University Collage, Bartella, Iraq.
Please see the book here:- https://doi.org/10.9734/bpi/psnid/v5/1665
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