Alarmingly Finding the Growth of Animal Cell (Mouse Fibroblast) in the Presence of Antinutrient Gossypol in Culture | Chapter 12 | Innovations in Biological Science Vol. 5

 

An experiment was conducted to investigate the effects of gossypol on growth of animal cells in culture. A cells culture was established according to the methods of Freshney (2010). Four treatments examined were To= cells + media, Te= cells + ethanol, Tgl= cells + 5 µM gossypol, and TgH= cells + 20 µM gossypol. The results of this experiment indicated that the presence of gossypol 5 µM inhibited (p<0.01) cells growth, and gossypol at 20 µM completely stopped the increased in the numbers of cells. It was concluded that the division and growth of animal cells in culture were adversely depressed by gossypol probably due to gossypol-protein complexes which enveloping cells, preventing protein transferred from media to cells, and reduced the nutrient availability for growth.

 

Author(s)details:-

 

Ismartoyo
Department of Animal Science, Hasanuddin University, Makassar-90245, Indonesia.

 

Please See the book here :- https://doi.org/10.9734/bpi/ibs/v5/577

Assessing the In Vitro Antioxidant Activity of Withania somnifera Root | Chapter 8 | Current Perspectives on Chemical Sciences Vol. 6

 Due to their structural sophistication, chemical diversity, lack of significant toxic effects and a wide range of antimicrobial activity, conventional herbal medicines are new therapeutic candidates. It can be found in the Mediterranean, Africa, and India. Objective: To test and compare the in vitro antioxidant activity of the indigenous Withania somnifera methanol extract to that of the imported root extract. Methods: ABTS•+ radical cation de-colorization assay, DPPH• radical scavenging activity, radical scavenging activity of nitric oxide, Fe2+ chelating activity assay, radical scavenging activity of superoxide anion and hydrogen peroxide and power reduction of ferrous were examined. Results: Comparatively stronger antioxidant activity was observed for indigenous root extracts than for imported root extracts. Conclusion: The indigenous root data obtained from these studies were high compared to the imported root extracts which showed higher antioxidant activity in Withania somnifera indigenous root. Thus, the isolation of Withania somnifera bioactive compounds would certainly serve as a good phytotherapeutic agent. Compared to the imported root extract, the indigenous root extract showed high antioxidant capacity. The present study of the antioxidant activities of Withania somnifera root in Bangladesh is the first ever paper.

Author(s) Details

Ratan Kumar Paul
Department of Chemistry, Pabna University of Science and Technology, Pabna, Bangladesh and Department of Chemistry, Bangladesh University of Engineering & Technology, Dhaka, Bangladesh.

Dali Rani Sarker
Department of Chemistry, Pabna University of Science and Technology, Pabna, Bangladesh.

Md. Saifur Rahaman
Institute of Radiation and Polymer Technology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh.

View Book :- https://bp.bookpi.org/index.php/bpi/catalog/book/355