Vinblastine, a Vinka alkaloid and a synthetic analogue of vincristine is generally secondhand in combination analysis with synthetic fragments. A new simple, exact and accurate reverse phase HPLC order was developed and ratified for the estimation of vinblastine in pharmaceutical formulations utilizing the mobile aspect 90% methanol, 5% acetonitrile and 5% of 0.1% orthophosphoric acid and the pH was maintained at 3.5. Reasoning was carried out utilizing PEAK 7000 isocratic HPLC with rheodyne manual sample injector with a switch (77251). The effect chromatogram obtained has a extreme resolution and low following factor (1.29). The extent of object curve showed a correlation cooperative (r2) of 0.999 for a wide range of drug concentrations of 3-15 ppm. The form was also validated in respect of accuracy, accuracy and particularity. The applicability of the method was too tested accompanying commercial sample of Cytoblastin. This method is more correct, precise and available in terms of linearity, veracity, precision, and so forth.
Author(s) Details:
P. Rama Krishna Veni,
Department
of Applied Sciences and Humanities, Sasi Institute of Technology &
Engineering, Tadepalligudem - 534101, Andhra Pradesh, India.
N.
Sharmila,
Department
of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur - 522510,
Andhra Pradesh, India.
B. Hari Babu,
Department of Chemistry, Acharya Nagarjuna University, Nagarjuna
Nagar, Guntur - 522510, Andhra Pradesh, India.
Please see the link here: https://stm.bookpi.org/ACPR-V4/article/view/13007
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