Aims:
The
pure stability of aspirin in the aspirin (100 μg/mL) only and aspirin (100
μg/mL)+curcumin(600 μg/mL) admixture without any
ingredient under two solvents (distilled water,
DW and normal saline, NS), three storage
temperatures(25ºC, 4ºC and -20ºC) and periods (1st,3rdand
7thdays) was evaluated.
Study
Design: The injectable DW- and NS-aspirin containing solutions in the laboratory
cap
polyethylene bottle were stored and evaluated at
controlled temperature (25ºC, 4ºC and -20ºC) during
7 days.
Methodology:
Effects
of admixture compounds, periods of storage and temperature of storage on the
concentrations of active compound (aspirin) were
analyzed. The concentration of aspirin in each
solution was determined by stability-indicating
high-performance liquid chromatography (HPLC)-
ultraviolet (UV) detection. A 1.0 mL volume of each
sample was withdrawn and reconstituted with 3.0
mL of ethanol and directly injected into HPLC system
immediately after filtration at 1st, 3rdand 7th days
for analysis. The stability of the solutions was
determined by calculating the percentage of the initial
aspirin concentrations remaining at each test condition
and periods. Stability was defined as the
retention of at least 90% of the initial aspirin
concentration.
Results:
The
concentration of aspirin of the aspirin only and aspirin+curcumin admixture
solutions
remained at least 90% of original without any color
change or precipitation in the DW and NS solution
at 4ºC and -20ºC throughout 7-day period and showed
instability that decreased gradually below 90%
of original concentrations after 1 day at 25ºC in the two
solutions.
Conclusion:
Two
kinds of solutions of only aspirin and aspirin+curcumin admixture, in DW and
NS,
showed different stability dependence on temperature of
storage that means maintained stability at
4ºC and -20ºC and did not show effect of admixture of
curcumin on aspirin stability during 7 days
except 25ºC.
Author(s) Details
Hyun-Jin Kim
Department of Pharmacology and Clinical Pharmacology Laboratory, College
of Medicine, Hanyang University, Seoul 04763, South Korea.
Ju-Seop Kang
Department of Pharmacology and Clinical Pharmacology Laboratory, College
of Medicine, Hanyang University, Seoul 04763, South Korea.
View Book :- http://bp.bookpi.org/index.php/bpi/catalog/book/222
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