Acute and Sub-chronic Toxicity Study of the Extract and Powder of Operculina macrocarpa (L.) Urb. in Mice: Recent Perspectives | Chapter 8 | Current Strategies in Biotechnology and Bioresource Technology Vol. 2

The aim of the study was to evaluate the experimental acute and sub-chronic toxicities of Operculina macrocarpa with plant extract and powder, respectively, in male and female mice. Phytochemical prospection was performed with extract and administration in single doses by intraperitoneal route   to six groups and control at 1230, 970, 700, 350, 120 and 30 mg/kg doses and distilled water   (0.1 ml/10 g), respectively. Sub-chronic doses of 1230, 700, 30 mg/kg/day and satellite (1230 mg/kg/day) were administered orally in feed. Major endpoints included alterations in the central and autonomic nervous system, water and food intake, body weight, hematological and biochemical parameters. Phytochemical screening identified compounds: Alkaloids, flavonoids, xanthones, leucoanthocyanidins and tannins condensate. In the acute study, mortality was observed with toxicity signs to the central nervous system (CNS) at LD50 of 270 mg/kg. There were no significant changes in water and food intake, body weight, haematological and biochemical parameters, and histopathological examination in the sub-chronic study (p value). Results indicate that the oral administration of O. macrocarpa powder in feed is less toxic and relatively safer.

Author(s) Details

Carpejane Ferreira da Silva
Department of Veterinary Medicine, Federal University of Campina Grande, UFCG, Patos, Paraíba, Brazil.

Ana Célia Rodrigues Athayde
Biological Sciences Unit, Federal University of Campina Grande, UFCG, Patos, Paraíba, Brazil.

Marcílio Fontes Cezar
Veterinary Medicine Unit, Federal University of Campina Grande, UFCG, Patos, Paraíba, Brazil.

Onaldo Guedes Rodrigues
Veterinary Medicine Unit, Federal University of Campina Grande, UFCG, Patos, Paraíba, Brazil.

Sergio Santos Azevedo
Biological Sciences Unit, Federal University of Campina Grande, UFCG, Patos, Paraíba, Brazil.

José Galberto Martins da Costa
Department of Biological Chemistry, Regional University of Cariri, Crato, Ceara, Brazil.

Fabrício Kleber de Lucena Carvalho
Department of Veterinary Medicine, Federal University of Campina Grande, UFCG, Patos, Paraíba, Brazil.

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