Introducing: M. Oleifera is a highly valued
medicinal plant that is widely used for the treatment of different ailments. In
many tropical & subtropical countries, it is found. The leaves have many
medicinal and nutritional values, with the phytochemicals responsible for the
medicinal values therein, and when consumed in large quantities, they cause
hepatotoxicity. Aim: To decide if N-acetyl cysteine (NAC) has a protective or
reversal effect on M. Induced-subacute hepatotoxicity of oleifera leaf aqueous
extract in Wistar albino rats. Methods: An experimental laboratory-based study
was performed at the University of Makerere, Department of Animal House
Physiology. On 48 Wistar Albino rats aged 8-10 weeks, 2 experiments (1) established
the protective effects and (2) established the reversal effects of NAC. There
were 3 community therapies for the protective effects. The standard saline (1
ml) + toxic dose of M was administered to group I. Group II obtained a toxic
dose of acetaminophen (750 mg / kg)+ NAC (50 mg / kg) for oleiferaleaf aqueous
extract (8.05 g / kg), and group III received M. (8.05 g / kg), + NAC (50 mg /
kg) oleifera. The doses were administered daily for 28 days using an oral
intragastric tube. Five treatment groups that produced subacute hepatotoxicity and obtained
varying doses of NAC and paracetamol for 7 days had reversal effects. Group 1
received 1 ml of regular saline; Group 2 received 1000 mg / kg of NAC; Group 3
received 1200 mg / kg of NAC; Group 4 received 1,500 mg of NAC; and Group 5
received 1,200 mg / kg of NAC with paracetamol-induced hepatotoxicity. On days
1, 3 and 7, liver enzymes (AST, ALT, and ALP), bilirubin and total serum
protein were determined. Both animals were slaughtered under oreuthanized
anaesthesia and their liver was extracted for histopathological analysis.
Results:
Treated animals with a mixture of NAC and a toxic dose of M. On average,
oleifera or paracetamol showed normal liver enzymes and levels of bilirubin.
NAC-treated animals after M. Hepatotoxicity-induced oleifera and paracetamol
displayed normal liver enzymes and levels of bilirubin. The histopathological
examination revealed moderate hepatitis characteristics. Conclusion: NAC
protections and overturns M. The aqueous extract-induced sub-acute
hepatotoxicity of oleifera leaf in Wistar Albino rats.
Author(s) Details
Josephine N. Kasolo
Department of Medical Physiology,
Makerere University College of Health Sciences; P.O.Box 7072, Kampala, Uganda.
Agnes Namaganda
Department of Medical Physiology,
Makerere University College of Health Sciences; P.O.Box 7072, Kampala, Uganda.
Dr. Godfrey S. Bbosa
Department of Pharmacology and Therapeutics, Makerere University College of
Health Sciences; P.O.Box 7072, Kampala,Uganda.
Haruna Muwonge
Department of Medical Physiology,
Makerere University College of Health Sciences; P.O.Box 7072, Kampala, Uganda.
Robert Lukande
Department of Pathology, Makerere
University College of Health Sciences; P.O.Box7072, Kampala, Uganda.
Dr. Joshua Nfambi
Department of Medical Physiology, Makerere University College of Health Sciences;
P.O.Box 7072, Kampala, Uganda.
Ivan Kimuli
Department of Medical Physiology,
Makerere University College of Health Sciences; P.O.Box 7072, Kampala, Uganda.
Katamba Godfrey
Department of Medical Physiology,
College of Health Sciences, King Caesar University, Kampala, Uganda.
Isaac Okullo
Department of Dental Surgery,
Makerere University College of Health Sciences; P.O.Box 7072, Kampala, Uganda.
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