Beneficial Effects of Nigella sativa for Osteoporosis and Bone Healing | Chapter 1 | Challenges and Advances in Pharmaceutical Research Vol. 8

 Osteoporosis is the most common cause of bone fracture in the senior. Several experimental studies have reported that Nigella sativa( NS) and/ or its main element thymoquinone( TQ) have good goods on osteoporosis and bone mending. We conducted this methodical review to estimate these applicable studies and prove whether NS and/ or TQ have a implicit effect on osteoporosis and can halt the pathogenesis of this complaint or whether this is just a fabrication. A hunt for published studies was conducted in databases similar as Scopus, PubMed, Google Scholar, Web of Science and CINAHIL. The hunt terms included" Nigella sativa, black seed, TQ, osteoporosis, bone mending". originally, 213 papers were uprooted. After reviewing the titles and objectifications, 124 papers( Medline, 43; Scopus, 67; EBSCO, 14) were named for farther analysis. After banning clinical trial studies, reviews, abstract junking and unconnected studies, seven studies were eventually considered suitable for our review. Eventually, seven studies were considered suitable for our review. The total number of creatures used was 220( 150 rats and 70 rabbits) from different experimental studies. Grounded on the results of this methodical review, we conclude that NS or remedy with TQ excerpts can not yet be recommended for osteoporosis and that these data aren't sufficient to reliably rule out the salutary goods of NS on bone development. The α- form of the oestrogen receptor appears to be most important in regulating bone development. thus, farther studies are demanded to probe the specific cellular and molecular targets of NS or TQ using osteoporosis beast models.

Author(s) Details:

Muhamed T. Osman,
Faculty of Medicine and Health Sciences, UCSI University, 2, Avenue, 3, Persiaran Springhill, 71010, Port Dickson, Negeri Sembilan, Malaysia.

Please see the link here: https://stm.bookpi.org/CAPR-V8/article/view/8489

Assessment of RP-HPLC Method Development and Validation for Simultaneous Estimation of Thymoquinone and Curcumin in Dosage Form| Chapter 14 | Challenges and Advances in Pharmaceutical Research Vol. 2

The goal of this research is to create and verify a simple, specific, accurate, and exact HPLC technique for concurrently measuring Curcumin (CUR) and Thymoquinone (THQ) in bulk and formulation, following ICH guidelines for analytical method development and validation.

Study Design: Using a C-18 Inertsil column and optimising factors, develop an RP-HPLC technique for quantifying Thymoquinone and Curcumin in bulk and formulation.

The current research was carried out in the year 2021 at Shri D. D. Vispute College of Pharmacy and Research Center, Panvel.

The RP-HPLC technique was developed employing an isocratic mobile phase of acetonitrile and water at a ratio of (82:18) v/v, with a flow rate of 0.9 mL/minute over an Inertsil ODS, 250 4.6 mm, 5 m column, at a column oven temperature of 30°C. A 256 nm photodiode array was used for detection.

The retention periods for curcumin and thymoquinone were 3.5 and 4.3 minutes, respectively. The technique displayed good linear response for thymoquinone and curcumin in concentration ranges of 4-18 g/ml and 10-45 g/ml, respectively, with correlation coefficient (R2) values of 0.999 for both. The highest permissible limit percentage of relative standard deviation for curcumin and thymoquinone studies was less than 2.0, i.e. 1.61 percent and 1.62 percent for curcumin and 0.47 percent and 0.42 percent for thymoquinone, respectively. The mean % recovery for both drugs was within acceptable limits. The devised and verified HPLC technique is simple, accurate, precise, and acceptable for analysis since all of the findings meet the acceptance criteria.

Conclusion: The new RP-HPLC technique at single wavelength was validated for system appropriateness, specificity, linearity, accuracy, precision, and robustness according to ICH requirements, and may be used for regular quality monitoring of medicines in pharmaceutical dosage forms. This approach may be used to analyse curcumin and thymoquinone samples together for routine quality control.

 

Author(s) Details:

Prajakta Jagtap,
Department of Quality Assurance, Shri. D. D. Vispute College of Pharmacy & Research Center, New Panvel - 410206, India.

Namrata Mahajan,
Department of Quality Assurance, Shri. D. D. Vispute College of Pharmacy & Research Center, New Panvel - 410206, India.

Anjali Parte,
Department of Quality Assurance, Shri. D. D. Vispute College of Pharmacy & Research Center, New Panvel - 410206, India.

Jeeja Pananchery,
Department of Pharmacognosy, Shri. D. D. Vispute College of Pharmacy & Research Center, Ne

Ashish Jain,
Department of Pharmacognosy, Shri. D. D. Vispute College of Pharmacy & Research Center, New Panvel - 410206, India.

Please see the link here: https://stm.bookpi.org/CAPR-V2/article/view/6586

Cold Press Black Seed Oil and Omega-3 and Its Beneficial Effects on Metabolic Function and Fatty Liver in Obese Model | Chapter 6 | Current Research in Agricultural and Food Science Vol. 3

 Aim: A high amount of Thymoquinone (TQ), an antioxidant, is present in Nigella Sativa seeds. Therefore, we hypothesised that Nigella Sativa oil will boost obesity-induced hyperglycemia through the antioxidant properties of TQ, and decrease blood pressure and OX-LDL in obese mice. The goal of this study is to examine the effects of TQ and omega-3 PUFAs (polyunsaturated fatty acids) on inflammation associated with obesity, resistance to insulin and the metabolic effects of browning of adipose tissue.

Methods: 3T3-L1 cells, fat adipocytes, were cultured to investigate the effects of TQ and omega-3 on WAT browning. For 20 weeks, C57B16 male mice were fed a high fat diet (HF) beginning at eight weeks of age. Mice were divided into four treatment groups of five animals, each according to the following: group 1) Lean, group 2) HF diet, group 3) HF diet treated with 3% TQ for the last 8 weeks, and group 4) with 3% omega-3. Blood biomarkers, antioxidant biomarkers, mitochondrial activity and accumulation of tissue fat have been measured.
Results: Increased energy-dependent genes and oxygen consumption resulted in 3 percent TQ treatment (P<0.05). Compared to CA gasoline, TQ lowered fasting glucose and blood pressure (P<0.05) levels. TQ increased hepatic HO-1, mitochondrial Mfn2, PGC1aa, phosphorylation of the insulin receptor and decreased OX-LDL (P<0.05) and haptic apoptosis levels. The size of lipid droplets and increased hallmarks of beige adipocyte proteins such as UCP1, PRDM16, FGF21, Sirt1, Mfn2 and HO-1 protein expression, as well as elevated insulin receptors, were reduced by fat adipocytes, 3T3-L1 cells, TQ and omega-3. Treatment with TQ and omega-3 in the adipose tissue of HFD mice attenuated levels of inflammatory adipokines, beige adipocyte hallmarks and mitochondrial biogenesis markers. TQ and omega-3 have also increased inflammation and insulin sensitivity in the liver and decreased the expression of inflammatory genes in addition to adipose. Our data show that TQ therapy in combination with omega-3 can play an important role in reducing the fatty liver associated with obesity, insulin resistance and the chronic inflammatory condition of obesity.

Author (s) Details

Maria Licari
Department of Medicine, New York Medical College, Valhalla, New York 10595, USA and Department of Pharmacology, New York Medical College, Valhalla, New York 10595, USA and Department of Drug Sciences, University of Catania, Catania, Italy.

Marco Raffaele
Department of Pharmacology, New York Medical College, Valhalla, New York 10595, USA and Department of Drug Sciences, University of Catania, Catania, Italy.

Lars Bellner
Department of Pharmacology, New York Medical College, Valhalla, New York 10595, USA.

Luca Vanella
Department of Drug Sciences, University of Catania, Catania, Italy.

Rita Rezzani
Anatomy and Physiopathology Division, Department of Clinical and Experimental Sciences, University of Brescia, Brescia, Italy.

Francesca Bonomini
Anatomy and Physiopathology Division, Department of Clinical and Experimental Sciences, University of Brescia, Brescia, Italy.

Hsin Hsueh Shen
Department of Pharmacology, New York Medical College, Valhalla, New York 10595, USA and Department and Institute of Pharmacology, National Defense Medical Center, Taipei, Taiwan.

Stephen J. Peterson
Department of Medicine, Weill Cornell Medicine, New York, NY 10065, USA and New York Presbyterian Brooklyn Methodist Hospital, Brooklyn, NY 11215, USA.

Nader G. Abraham
Department of Medicine, New York Medical College, Valhalla, New York 10595, USA and Department of Pharmacology, New York Medical College, Valhalla, New York 10595, USA.

View Book :- https://bp.bookpi.org/index.php/bpi/catalog/book/364