Ovine Pulmonary Adenocarcinoma in Libya: The First In-Depth Gross and Histopathological Investigation | Chapter 13 | Microbiology and Biotechnology Research: An Overview Vol. 4

 

Background: Ovine pulmonary adenocarcinoma (OPA) is a contagious, neoplastic lung disease of sheep that causes significant animal welfare and economic issues throughout the world.

 

Aims: The study aims to provide a preliminary estimate of the prevalence of  OPA among slaughtered sheep in eastern Libya and to delineate the morphopathological characteristics of OPA in native Libyan sheep.

 

Methods: This study was carried out in the El-Beida slaughterhouses located in Eastern Libya during October 2020- March 2023. The lungs of 641 sheep carcasses were examined. The gross examination of these lungs was carried out with respect to size, colour and consistency. The lungs with gross lesions (n = 322) were collected and subjected to histopathological examination.

 

Results: In 1.56% of total carcasses examined and in 3% of abnormally appearing lungs, OPA lesions were discovered. The classical form was present in 8 of 10 confirmed cases of OPA, and two presented with atypical lesions. The disease manifests through progressive transformation of the pulmonary epithelium, and the histopathological lesions in both forms were generally comparable and comprised typical neoplastic proliferations of alveolar and bronchiolar epithelium with inflammatory infiltration of variable degrees.

 

Conclusion: This report confirms the existence of OPA in the native sheep of the El-Beida area, with the occurrence of both the classical and the atypical types. The morphopathological findings form useful diagnostic references for standard pathological examination and emphasise the imperative for further investigations on the epidemiology, etiologic factors, and pathogenesis of OPA in Libyan sheep populations.

 

Author(s) Details

Zahra M. A. Mohammed
Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Omar Al-Mukhtar niversity, El-Beida, Libya.

 

Please see the book here:- https://doi.org/10.9734/bpi/mbrao/v4/5558

Determination of Serological Data to Understand the Epidemiology of Foot-and-mouth Disease Virus Serotypes Circulating in Libya | Chapter 01 | Innovations in Microbiology and Biotechnology Vol. 5

The third of these components, measuring the level of NSP antibodies as an indicator of previous or current FMDV infection, regardless of vaccination status, was the subject of this investigation. For nearly fifty years, outbreaks of foot-and-mouth disease (FMD) have been rare in Libya. In the spring of 2013, a nationwide serosurvey was done to determine the level of FMD viral circulation and transmission  determine the serotypes of the FMD virus in the nation A total of 4221 sera were obtained, with samples from all around the world.small ruminants (SR; samples from 357 farms) and large ruminants (LR; samples from 357 farms 141 farms were sampled). By ELISA, the FMD sero-prevalence of NSP antibodies was 19.0 percent.  (271/1428) with a 95 percent confidence interval of (16.9 - 21.0) and 13.5 percent (378/2793) with a confidence interval of (12.3 - 14.8) for LR and SR, respectively. samples, and so forth In LR, the sero-prevalence of NSP antibodies was and was age-related.  While in SR, the group year and year were respectively, and year, year, and year, correspondingly, for age groups year, year, and year. These The NSP serologic profiles revealed support the idea of endemic FMD circulation in the area. Libya. All positive samples were screened for SP antibodies against the FMD virus serotypes O, A, and SAT-2. The most common circulating serotype was serotype O, followed by serotype A, with no evidence of SAT-2. was not discovered. These findings shed light on the overall epidemiology of FMD in Libya, and contribute to field and lab investigations of serotype 0 (O/ME-SA/Ind-2001) in 2013 Clinical samples from around the country were used to isolate the lineage. in order to gain a better grasp more information about livestock production, data collection for the management system, and risk factor analysis It is necessary to conduct research. Scientists will be able to better grasp the epidemiology of the disease as a result of the findings. FMD and develop scientifically sound disease control measures to lessen the impact of FMD on people and the environment. Livestock from Libya.

Author (s) Details

Ibrahim Eldaghayes
Faculty of Veterinary Medicine, University of Tripoli, P. O. Box 13662, Tripoli, Libya.

Abdunaser Dayhum
Faculty of Veterinary Medicine, University of Tripoli, P. O. Box 13662, Tripoli, Libya .


Abdulwahab Kammon
Faculty of Veterinary Medicine, University of Tripoli, P. O. Box 13662, Tripoli, Libya.

Monier Sharif
Faculty of Veterinary Medicine, University of Omar Al-Mukhtar, Albeida, Libya.

Giancarlo Ferrari
Food and Agriculture Organization of the United Nations (FAO), Rome, Italy.

Christianus Bartels
European Commission for the Control of Foot-and-Mouth Disease (EuFMD), Food and Agriculture Organization of the United Nations (FAO), Rome, Italy.

Keith Sumption
European Commission for the Control of Foot-and-Mouth Disease (EuFMD), Food and Agriculture Organization of the United Nations (FAO), Rome, Italy.

Donald P. King
The Pirbright Institute, Ash Road, Surrey, UK.


Santina Grazioli
Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER), Brescia, Italy.


Emiliana Brocchi
Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER), Brescia, Italy.


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Isolation and Molecular Identification of Vibrio spp. by Sequencing of 16S rDNA from Seafood, Meat and Meat Products in Libya: A Descriptive Study | Chapter 10 |Innovations in Microbiology and Biotechnology Vol.4

The study's major goal was to define microorganisms obtained from seafood, meat, and meat products that could cause food poisoning. We intend to use this information to establish a baseline for future foodborne illness studies in Libya. Septicemia, cholera, and milder forms of gastroenteritis are all caused by food-borne microbes in the genus Vibrio. Vibrio cholerae, Vibrio parahemolyticus, and Vibrio vulnificus are some of the Vibrio species that are commonly associated with foodborne transmission. A total of 93 samples of seafood, meat, and meat products (Table 1) were randomly collected from different geographic locations in Libya [Tripoli, Regdalin (120 km west of Tripoli), Janzour (30 km west of Tripoli), and Tobruk (1400 km east of Tripoli)]: 21 shrimps, 5 clams, 20 fish, 34 samples of raw meat (10 beef, 9 camel meat, 6 mutton, and 9 chickens), and 13 samples of meat products (2 beef sausages, 5 Each sample weighed 250 grammes. Only 48 (51.6%) of the 93 cultivated samples developed colonies on Thiosulfate Citrate Bile Salt agar (TCBS) with Vibrio spp. culture characteristics. On TCBS, more than half (n=27) of processed seafood samples (n=46) yielded colonies, whereas only 44.6 percent of meat and meat product samples yielded colonies. The highest bacterial count among cultivated seafood samples was found in clams, which had a level of 3.8X104 CFUg. With 6.5X104 CFUg, chicken burger samples had the highest bacterial count. Molecular examination of the isolates acquired in this investigation revealed that Vibrio spp. were found in 11 of the 48 samples (22.9 percent). For the first time, Vibrio parahemolyticus was isolated from camel meat. Finally, morphological features extracted from morphological cultural characteristics on the selected TCBS agar media were used to presumptively identify the retrieved Vibrio parahemolyticus and V. harveyi.

Author(s) Details:

S. M. Azwai,
Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, P.O. Box-13662, Tripoli, Libya.



E. A. Alfallani,
Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, P.O. Box-13662, Tripoli, Libya.


S. K. Abolghait,
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Suez Canal University, Ismailia-41522, Egypt.


A. M. Garbaj,
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Tripoli, P.O. Box-13662, Tripoli, Libya.


H. T. Naas,
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Tripoli, P.O. Box-13662, Tripoli, Libya.


A. A. Moawad,
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, Giza-12211, Egypt.


F. T. Gammoudi,
Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, P.O. Box-13662, Tripoli, Libya.


H. M. Rayes,
Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, P.O. Box-13662, Tripoli, Libya.


I. Barbieri,
Istituto Zooprofilattico Sperimentale della Lombardia e dell’ Emilia Romagna, Via Bianchi, 9 - 25124 Brescia, Italy.


I. M. Eldaghayes,
Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, P.O. Box-13662, Tripoli, Libya.

Please see the link here: https://stm.bookpi.org/IMB-V4/article/view/6230

The History and Prevalence of Cystic Hydatidosis (Hydatid Disease) in Sheep, Goats, Cattle and Camels in Libya: A Review | Chapter 7 | Research Aspects in Agriculture and Veterinary Science Vol. 3

Cystic hydatid disease is caused by Echinococcus granulosus, a cestode parasite. Hydatid cysts are one of the most common parasitic infections in Libya, causing a variety of health problems in humans and financial losses due to the condemnation of infected viscera from slaughtered animals, as well as a reduction in the quality and quantity of other livestock products like milk, wool, and meat. The parasite's adult stage is about 3-7 mm long, with three segments on average, as well as other morphological and morphological features that aid in species identification. According to many abattoir examinations in Libya, cystic hydatidosis is a disease that affects a variety of animal species with varying rates of infection, including sheep (1.6 to 40%), goats (5.6 to 70%), cattle (2.7 to 56%), and camels (2.7 to 48%). (2.7 to 48 percent ). Based on available abattoir data, cystic echinococcosis in cattle can be classified as highly endemic across the country, with infection rates of about 50% or more in all animal species, meeting WHO requirements. Because all abattoirs lack reliable and up-to-date government records, determining the exact financial losses caused by cystic hydatidosis in livestock is challenging. The diagnosis of the parasite larval stage (hydatid disease) in living intermediate hosts (ante mortem) is primarily based on imaging and serological methods, whereas the post mortem examination of hydatid cysts can be based on inspection of several expected infected organs, primarily the liver and lungs, but also the spleen, kidneys, heart, brain, and bones of the animal. Veterinary efforts on reducing the extent and intensity of echinococcosis in definitive host populations, which may indirectly lead to control of hydatid disease prevalence in intermediate host species, are the mainstays of cystic hydatid disease prevention. Animals with cystic hydatidosis can be treated with anti-helminthic drugs, though this is currently being researched. In order to limit the level of infection and egg emission, regular treatment, extreme caution when handling pets or dealing with animal flesh, and immunisation of ruminant intermediate hosts are all being examined.

Author(S) Details

Mohamed M. Rhagem
Department of Zoology, Faculty of Sciences, University of Zawia, P.O.Box 16418, Zawia, Libya.

Wafa M. Ibrahem
Department of Parasitology, Faculty of Medicine, University of Zawia, P.O.Box 16418, Zawia, Libya.

Kawther M. Ibrahem
Department of Medicinal Chemistry, Faculty of Pharmacy, University of Zawia, P.O.Box 16418, Zawia, Libya.

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