Microbial Profiling, Bioremediation of Cadmium and Dye Decolourization Competence of Marine Bacterial Isolates | Chapter 11 | New Innovations in Chemistry and Biochemistry Vol.7

 The microbial community is one of the many varied groups found in the ocean. Studies have showed that microorganisms isolated from the marine environment are metabolically active and have adapted to life in the water. To battle heavy metal stress and decolorization of diverse textile dyes, marine microorganisms employ a variety of survival strategies, and hence play an essential role in cadmium bioremediation and textile dye degradation. The current research focuses on the isolation and 16S rRNA molecular characterisation of M3, M8 bacterial strains recovered from Visakhapatnam port marine water samples. The potential of M3 and M8 isolates to remove cadmium from the environment and decolorize various textile dyes was also examined. The tube dilution method was employed to isolate bacterial strains from the water sample, and 10 distinct isolates were examined. To identify the species, biochemical tests were performed on the isolates, as well as 16S rRNA molecular sequencing and phylogenetic analysis. Bacillus subtilis and Pseudomonas resinovorans were identified as Bacillus subtilis and Pseudomonas resinovorans, respectively, after 16srRNA molecular sequencing and phylogenetic analysis. The two bacterial strains showed promising dye degradation characteristics as well as removal of cadmium from the growing media when tested with nine different textile dyes with wavelengths ranging from 400 to 600nm. The isolates M3 and M8 are shown to be potential strains for dye decolorization and cadmium bioremediation in the current investigation.

Author(s) Details:

M. Teja,
Department of Biotechnology, Andhra University, Visakhapatnam-530003, India.

D. Muni Kumar,
Department of Biochemistry, Andhra University, Visakhapatnam-530003, India.

P. Bindiya,
Department of Biotechnology, Andhra University, Visakhapatnam-530003, India.

G. Sudhakar,
Department of Human Genetics, Andhra University, Visakhapatnam-53003, India.

Please see the link here: https://stm.bookpi.org/NICB-V7/article/view/5927

Study on Isolation and Characterization of Crude oil Degrading Bacteria from Oil Spill Sites of Ennore Port | Chapter 7 | New Visions in Biological Science Vol. 1

 The goal of this study was to isolate and identify bacterial strains as well as assess their crude oil biodegradation capacity in vitro. Oil spills are a major cause of pollution and a constant threat to the maritime environment. Crude oil degrading microbes were isolated and identified using 16S rRNA gene sequencing to solve the problem. Under in vitro settings, a preliminary investigation was conducted to assess the potential of local bacterial strains to use crude oils as the sole carbon source. The oil breakdown efficiency of seven isolated bacterial strains from the oil spill impacted areas was tested. Using selected bacterial cultures, biodegradation studies were carried out in 1% crude oil for 12 days. Only three of the seven isolates, P. aeruginosa (AMETOS02), P. stuartii (AMETOS01 & AMETOS03), showed the highest oil degradation potential. P. stuartii (AMETOS03) shown potential biosurfactant activity, and the kind of hydrocarbon degraded was investigated further using gas chromatography–mass spectrometry (GC-MS). Our findings suggest that P. stuartii (AMETOS03) is a viable candidate for bioremediation of crude oil (hydrocarbon).

Author (S) Details

Gokulalakshmi Elayaperumal
Human Genetics Laboratory, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India.

M. Vanitha Chinnathambi
Department of Biotechnology, Guru Nanak College Chennai, Tamil Nadu, India.

K. Ramalingam
Centre for Marine Exploration, Department of Marine Biotechnology, AMET University, Kanathur, Chennai-603112, India.

View Book :- https://stm.bookpi.org/NVBS-V1/article/view/3175

Study on Isolation and Characterization of Crude oil Degrading Bacteria from Oil Spill Sites of Ennore Port | Chapter 7 | New Visions in Biological Science Vol. 1

 The goal of this study was to isolate and identify bacterial strains as well as assess their crude oil biodegradation capacity in vitro. Oil spills are a major cause of pollution and a constant threat to the maritime environment. Crude oil degrading microbes were isolated and identified using 16S rRNA gene sequencing to solve the problem. Under in vitro settings, a preliminary investigation was conducted to assess the potential of local bacterial strains to use crude oils as the sole carbon source. The oil breakdown efficiency of seven isolated bacterial strains from the oil spill impacted areas was tested. Using selected bacterial cultures, biodegradation studies were carried out in 1% crude oil for 12 days. Only three of the seven isolates, P. aeruginosa (AMETOS02), P. stuartii (AMETOS01 & AMETOS03), showed the highest oil degradation potential. P. stuartii (AMETOS03) shown potential biosurfactant activity, and the kind of hydrocarbon degraded was investigated further using gas chromatography–mass spectrometry (GC-MS). Our findings suggest that P. stuartii (AMETOS03) is a viable candidate for bioremediation of crude oil (hydrocarbon).

Author (S) Details

Gokulalakshmi Elayaperumal
Human Genetics Laboratory, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India.

M. Vanitha Chinnathambi
Department of Biotechnology, Guru Nanak College Chennai, Tamil Nadu, India.

K. Ramalingam
Centre for Marine Exploration, Department of Marine Biotechnology, AMET University, Kanathur, Chennai-603112, India.

View Book :- https://stm.bookpi.org/NVBS-V1/article/view/3175