Objective: The
objective of the study was to develop and UV Spectrophotometric method and
apply the method to dosage form.
Methods: A
simple, precise and sensitive ultraviolet spectrophotometric method was
developed for the determination of Fisetin in pure and pharmaceutical capsule
dosage form; the spectroscopic method was run through Shimadzu UV-1800 with
solvent of Methanol: 0.1%OPA was used in this method-working wavelength was
selected at 362nm.
Results:
Beer-Lambert’s law revealed a good correlation in the concentration range of
3-15µg/ml. The absorbance was found to be 0.385 with %RSD for interday
precision and intraday Precision was 0.53% & 0.51%.
Conclusion: The
developed method was successfully applied to the determination of Fisetin in
commercially available dosage forms. A statistical comparison of the results
showed an insignificant difference between the proposed method and reference
method. The proposed methods offered the advantages of simplicity and economy
that can be applied without the need for expensive instrumentation and reagents
in quality control analysis.
Author(s)details:-
R. Nageswara Rao
Department of Pharmaceutical Analysis, Santhiram College of Pharmacy,
Nandyal-518501, Andhra Pradesh, India.
L. Siva Shankar Reddy
Department of Pharmaceutical Analysis, Santhiram College of Pharmacy,
Nandyal-518501, Andhra Pradesh, India.
N. Madangopal
Department of Pharmaceutical Analysis, Santhiram College of Pharmacy,
Nandyal-518501, Andhra Pradesh, India.
M Lakshmi Devi
Department of Pharmaceutical Analysis, Santhiram College of Pharmacy,
Nandyal-518501, Andhra Pradesh, India.
R. Dharani
Department of Pharmaceutical Analysis, Santhiram College of Pharmacy,
Nandyal-518501, Andhra Pradesh, India.
K. Raj Kumar
Department of Pharmaceutical Analysis, Santhiram College of Pharmacy,
Nandyal-518501, Andhra Pradesh, India.
N. Venkateswara Reddy
Department of Pharmaceutical Analysis, Santhiram College of Pharmacy,
Nandyal-518501, Andhra Pradesh, India.
P. Chandana
Department of Pharmaceutical Analysis, Santhiram College of Pharmacy,
Nandyal-518501, Andhra Pradesh, India.
V. Ravikumar
Department of Pharmaceutical Biotechnology, School of Pharmacy, Guru Nanak
Institutions Technical Campus, Hyderabad, Telangana, India.
J. Kumar Raja
Department of Pharmaceutical Analysis, Mother Teresa College of Pharmacy,
Kothuru, Sanketika Nagar, Sathupally, Telangana 507303, India.
Please See the book
here :- https://doi.org/10.9734/bpi/prrat/v2/563
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