Background: The parasitic disease toxoplasmosis is brought on by the compilation protozoan agent Toxoplasma gondii and can result in substantial losses in terms of quality-adjusted life years and monetary damages. Consuming raw or overcooked meat has been a major source of transmission.
Aim: The present
study was conducted to survey the frequency of T. gondii in livestock meat
(sheep and goats) by using a MAT test and compare it with polymerase chain
reaction (PCR) methods as well as to determine the genetic diversity of
infecting strains of T. gondii.
Methods: This
cross-sectional study was conducted in Quchan city in Khorasan Razavi province
in northeastern Iran. Blood and tissue samples (diaphragm, heart) of 296
animals (including 168 sheep and 128 goats) were collected from the
slaughterhouse in Quechan City from August 2016 to April 2017. Serum samples
were examined by the Modified agglutination test (MAT) and the Nested-PCR
method performed to amplify the fragment of the B1 gene to detect parasite DNA
on tissues of seropositive animals. PCR-RFLP method of the GRA6 gene was used
to determine the genotype of T. gondii. Also, sequencing analysis was performed
to evaluate the Toxoplasma-type strains.
Results: Serum
positive for MAT results were found in 27.4% of Sheep and 23.4% of goats.
Positive Nested-PCR of the B1 gene results in diaphragm and heart tissues of
sheep and goats were 47.8% and 26.1%, 40% and 23.3%, respectively. Nested-PCR
of GRA6 gene results were positive in 10 samples that RFLP technique results using
MseІ enzyme revealed genotype І. Sequencing and Phylogenetic analysis revealed
DNA of all samples was closely related to Toxoplasma type І.
Conclusion: Given
the high seropositivity rate of toxoplasmosis, it is advised to implement a
suitable preventative program to lower the incidence of T. gondii infection
through cattle intake of raw or undercooked meat. Our research lends credence
to the idea that the intake of raw or undercooked meat by these animals may be
a potential source of toxoplasmosis in humans.
Seyedeh Nastaran
Asadzadeh
Department of Environmental Health, Environmental Health Engineering
Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.
Author(s)details:-
Hamed
Ghasemzadeh-Moghadam
Vector-Borne Diseases Research Center, North Khorasan University of Medical
Sciences, Bojnurd, Iran.
Mona Fani
Vector-Borne Diseases Research Center, North Khorasan University of Medical
Sciences, Bojnurd, Iran.
Mostafa Gholamrezaei
Department of Parasitology and Mycology, School of Medicine, Shahid
Sadoughi University of Medical Sciences, Yazd, Iran.
Nima Firouzeh
Vector-Borne Diseases Research Center, North Khorasan University of Medical
Sciences, Bojnurd, Iran.
Please See the book
here :- https://doi.org/10.9734/bpi/mria/v5/12387F
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