Pesticides find a potential application in agriculture,
combating pest and enhancing productivity. But on the reared, pesticides find
their way into aquatic habitats such as rivers, lakes and ponds, thereby
affecting aquatic flora and fauna. These pesticides cause significant variation
in esterase Isozyme patterns particularly in fish. The present investigation
was focused on the electrophoretic
banding patterns of esterase Isozyme in gill, liver, and brain tissues of the
freshwater fish Channa punctatus (Bloch) at different time intervals i.e. 24H,
48H, 72 H and 96 hrs after exposure of Malathion to those compared with control
group, the quantitative analysis of the esterase Isozymes were performed by
using 7.5 % of native polyacrylamide gel electrophoresis (PAGE) and stained
with α-naphthyl acetate as substrate. The relative motilities (Rm) of three
esterase isozyme bands in gill, liver, brain tissue were observed to be
0.60±0.05, 0.4±0.5. and 0.30±0.05 and bands were designated as Est-1, Est-2,
and Est-3, respectively. Compared to control group, both Est 2 and Est-3
esterase isozyme bands in gill and liver tissue were eliminated on exposure to
Author(s)details:-
Venkateswara Rao
Mandalapu
Department of Zoology, SR and BGNR Government Arts and Science College,
Khammam, Telangana-507002, India.
Venkaiah Yanamala
Department of Zoology, Kakatiya University, Warangal, Telangana State,
Telangana-506009, India.
Please See the book
here :- https://doi.org/10.9734/bpi/ibs/v5/8061E
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