Objective: The
present study highlights to develop and validate the RP-HPLC method and apply
the method to tablet dosage form.
Background: Most
human DNA polymerases are not affected and systemic toxicity of Lamivudine is
low. Various analytical methods have been reported for the estimation of
Lamivudine as alone as well as in combination with other drugs.
Method: A simple,
rapid, economical, precise and accurate RP-HPLC method for simultaneous
estimation of Lamivudine and Zidovudine in their combined dosage form has been
developed.
Results: A
reverse-phase high-performance liquid chromatographic method was developed for
the simultaneous estimation of Lamivudine and Zidovudine. Their combined dosage
form has been developed. The separation was achieved by LC- C18 column (150mm x
4.6mm, 5μm) and Water: Methanol (65:35v/v) as mobile phase, at a flow rate of
0.8 ml/min. Detection was carried out at 272 nm. The retention time of
Lamivudine and Zidovudine was found to be 3.007 min and 4.647, respectively.
The method has been validated for linearity, accuracy and precision. The assay
method was found to be linear from 50% to 150% for Lamivudine and Zidovudine.
Conclusion: The
developed method was found to be accurate, precise and rapid for the
simultaneous estimation of Lamivudine and Zidovudine in their combined dosage
form. The proposed method can be used for routine analysis of Lamivudine and
Zidovudine in combined tablet formulation.
Author(s)details:-
Bhoomi D.
Patel
Department of Pharmaceutical Chemistry & Quality Assurance, School of
Pharmacy, Rai University, Saroda, Dholka Road, Ahmedabad, Gujarat, India.
Mehta
Bhavya
Department of Quality Assurance, Saraswati Institute of Pharmaceutical
Sciences, Dhanap, Gandhinagar, Gujarat, India.
Ankit B.
Chaudhary
Department of Quality Assurance, Saraswati Institute of Pharmaceutical
Sciences, Dhanap, Gandhinagar, Gujarat, India.
Prakash H.
Kushwah
Department of Pharmaceutics, Varsha Goswami College of Pharmacy,
Ahmedabad, Gujarat, India.
Please See the book here :-https://doi.org/10.9734/bpi/prrat/v2/527
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