Increased
expression and activity of matrix metalloproteinase-2 (MMP-2) has been
correlated with an increase in invasive potential and a worse prognosis in many
cancers including melanomas. The antitumorigenic potential of all-trans
retinoic acid (ATRA) in melanomas was studied using the highly metastatic
murine melanoma cell line B16F10 as a model. Treatment of B16F10 cells with 20
µM/ml ATRA for 24 hrs caused a significant downregulation of MMP-2 expression
and activity. Inhibition of MMP-2 activity appeared to be mediated via a
significant decrease in expression of membrane type-1 matrix metalloproteinase
(MT1-MMP), a crucial component of the MMP-2 activation complex, and
extracellular matrix metalloproteinase inducer (EMMPRIN) and a significant
increase in expression of tissue inhibitor of metalloproteinases-2 (TIMP-2).
Downregulation of MMP-2 expression and activity would appreciably lower the
invasive potential of melanoma cells. Inhibition of phosphorylation of focal
adhesion kinase (FAK) upon ATRA treatment could inhibit intracellular
signalling cascades and also downregulate MMP-2 levels and cell migration. The
effectiveness of ATRA in inhibiting the migratory capabilities of B16F10 cells
was confirmed by wound healing assay. Our findings thus elucidate important
aspects about the molecular mechanisms of the effect of ATRA treatment on MMP-2
in melanomas and also indicate the anti-tumorigenic potential of ATRA which
could have therapeutic value in clinical management of invasive melanomas.
Author(s) Details
Dr. Aniruddha Banerji
Post Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata, 30 Mother Teresa Sarani, Kolkata 700016, West Bengal, India.
Dr. Kirat Kumar Ganguly
Department of Microbiology, Michael Madhusudan Memorial College, Durgapur 713216, West Bengal, India.
Dr. Amitava Chatterjee
Faculty Centre of ‘Integrated Rural Development and Management’, Ramakrishna Mission Vivekananda Educational and Research Institute, Narendrapur, Kolkata 700103, West Bengal, India.
View Book :- http://bp.bookpi.org/index.php/bpi/catalog/book/206
Author(s) Details
Dr. Aniruddha Banerji
Post Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata, 30 Mother Teresa Sarani, Kolkata 700016, West Bengal, India.
Dr. Kirat Kumar Ganguly
Department of Microbiology, Michael Madhusudan Memorial College, Durgapur 713216, West Bengal, India.
Dr. Amitava Chatterjee
Faculty Centre of ‘Integrated Rural Development and Management’, Ramakrishna Mission Vivekananda Educational and Research Institute, Narendrapur, Kolkata 700103, West Bengal, India.
View Book :- http://bp.bookpi.org/index.php/bpi/catalog/book/206
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