Determination of Phytochemicals as Potential Inhibitors of Matrix Metalloproteinases (MMPs) with Special Reference to Breast Cancer | Chapter 7 | Issues and Development in Health Research Vol. 5

Breast cancer is the most frequent cancer in women in India and around the world. Metastasis and secondary tumour growth are the leading causes of mortality and poor clinical prognosis in most malignancies, including breast tumours, and metastasized cancers remain essentially untreatable even with modern therapeutic options. MMPs, a family of zinc-dependent endopeptidase enzymes, play an important role in the regulation of metastasis and tumour formation, making them potential therapeutic targets for preventing metastasis. Therapeutic using phytochemical substances has the potential to lessen treatment adverse effects while also lowering treatment costs. The goal of this study is to look at the potential of four different phytochemicals, resveratrol, theaflavin, curcumin, and all-trans retinoic acid, as MMP inhibitors, with a focus on breast cancer.

Author (S) Details

Anirban Roy

Post Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata, 30 Mother Teresa Sarani, Kolkata 700016, West Bengal, India.

Indira Chakraborty

Post Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata, 30 Mother Teresa Sarani, Kolkata 700016, West Bengal, India.

Aniruddha Banerji

Post Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata, 30 Mother Teresa Sarani, Kolkata 700016, West Bengal, India.

View Book :- https://stm.bookpi.org/IDHR-V5/article/view/3969

All-trans Retinoic Acid (ATRA), a Potential Inhibitor of Matrix Metalloproteinase-2 (MMP-2) and Tumour Invasion in Melanomas | Chapter 14 | Current Strategies in Biotechnology and Bioresource Technology Vol. 2

Increased expression and activity of matrix metalloproteinase-2 (MMP-2) has been correlated with an increase in invasive potential and a worse prognosis in many cancers including melanomas. The antitumorigenic potential of all-trans retinoic acid (ATRA) in melanomas was studied using the highly metastatic murine melanoma cell line B16F10 as a model. Treatment of B16F10 cells with 20 µM/ml ATRA for 24 hrs caused a significant downregulation of MMP-2 expression and activity. Inhibition of MMP-2 activity appeared to be mediated via a significant decrease in expression of membrane type-1 matrix metalloproteinase (MT1-MMP), a crucial component of the MMP-2 activation complex, and extracellular matrix metalloproteinase inducer (EMMPRIN) and a significant increase in expression of tissue inhibitor of metalloproteinases-2 (TIMP-2). Downregulation of MMP-2 expression and activity would appreciably lower the invasive potential of melanoma cells. Inhibition of phosphorylation of focal adhesion kinase (FAK) upon ATRA treatment could inhibit intracellular signalling cascades and also downregulate MMP-2 levels and cell migration. The effectiveness of ATRA in inhibiting the migratory capabilities of B16F10 cells was confirmed by wound healing assay. Our findings thus elucidate important aspects about the molecular mechanisms of the effect of ATRA treatment on MMP-2 in melanomas and also indicate the anti-tumorigenic potential of ATRA which could have therapeutic value in clinical management of invasive melanomas.

Author(s) Details
Dr. Aniruddha Banerji
Post Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata, 30 Mother Teresa Sarani, Kolkata 700016, West Bengal, India.

Dr. Kirat Kumar Ganguly
 Department of Microbiology, Michael Madhusudan Memorial College, Durgapur 713216, West Bengal, India.

Dr. Amitava Chatterjee
Faculty Centre of ‘Integrated Rural Development and Management’, Ramakrishna Mission Vivekananda Educational and Research Institute, Narendrapur, Kolkata 700103, West Bengal, India.

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