Endogenous Regulators of Matrix Metalloproteinase Expression and Activity in Breast Cancers | Chapter 5 | Novel Research Aspects in Medicine and Medical Science Vol. 5

 Tumour often major and secondary tumour formation are the superior causes behind breast malignancy mortality. Modulation of matrix metalloproteinase (MMP) verbalization and activity promotes tumour often major and secondary tumour establishment in numerous cancers including feelings cancers. Extracellular matrix metalloproteinase inducer (EMMPRIN) and membrane type-1 cast metalloproteinase (MT1-MMP) are endogenous fragments which regulate MMP verbalization and activity. Our current study analyzes the importance of EMMPRIN (also known as Basigin or CD147) and MT1-MMP (MMP-14) accompanying particular reference to their roles in managing MMP expression and exercise in breast cancers. MT1-MMP and EMMPRIN expression levels are raised in invasive breast cancers and pertain an invasive phenotype and a poor prognosis by various devices including by causing an increase in MMP verbalization and activity. MT1-MMP likewise plays an important role in localization of MMP-2 to the tumour container surface, regulating proteolysis at the cell-ECM connect. Determination of MT1-MMP and EMMPRIN expression levels manage act as possible biomarkers for feelings cancers and may help clinicians to better diagnose the entertainment industry of the cancer. Therapies including inhibition of MMP expression and project to downregulate invasive potential in breast cancers take care of thus include the targeting of not just MMPs but also of their inside inhibitors EMMPRIN and MT1-MMP for increased adeptness.

Author(s) Details:

Anirban Roy,
Post Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata, 30 Mother Teresa Sarani, Kolkata 700016, West Bengal, India.

Aniruddha Banerji,
Post Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata, 30 Mother Teresa Sarani, Kolkata 700016, West Bengal, India.

Please see the link here: https://stm.bookpi.org/NRAMMS-V5/article/view/12165

All-trans Retinoic Acid (ATRA), a Potential Inhibitor of Matrix Metalloproteinase-2 (MMP-2) and Tumour Invasion in Melanomas | Chapter 14 | Current Strategies in Biotechnology and Bioresource Technology Vol. 2

Increased expression and activity of matrix metalloproteinase-2 (MMP-2) has been correlated with an increase in invasive potential and a worse prognosis in many cancers including melanomas. The antitumorigenic potential of all-trans retinoic acid (ATRA) in melanomas was studied using the highly metastatic murine melanoma cell line B16F10 as a model. Treatment of B16F10 cells with 20 µM/ml ATRA for 24 hrs caused a significant downregulation of MMP-2 expression and activity. Inhibition of MMP-2 activity appeared to be mediated via a significant decrease in expression of membrane type-1 matrix metalloproteinase (MT1-MMP), a crucial component of the MMP-2 activation complex, and extracellular matrix metalloproteinase inducer (EMMPRIN) and a significant increase in expression of tissue inhibitor of metalloproteinases-2 (TIMP-2). Downregulation of MMP-2 expression and activity would appreciably lower the invasive potential of melanoma cells. Inhibition of phosphorylation of focal adhesion kinase (FAK) upon ATRA treatment could inhibit intracellular signalling cascades and also downregulate MMP-2 levels and cell migration. The effectiveness of ATRA in inhibiting the migratory capabilities of B16F10 cells was confirmed by wound healing assay. Our findings thus elucidate important aspects about the molecular mechanisms of the effect of ATRA treatment on MMP-2 in melanomas and also indicate the anti-tumorigenic potential of ATRA which could have therapeutic value in clinical management of invasive melanomas.

Author(s) Details
Dr. Aniruddha Banerji
Post Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata, 30 Mother Teresa Sarani, Kolkata 700016, West Bengal, India.

Dr. Kirat Kumar Ganguly
 Department of Microbiology, Michael Madhusudan Memorial College, Durgapur 713216, West Bengal, India.

Dr. Amitava Chatterjee
Faculty Centre of ‘Integrated Rural Development and Management’, Ramakrishna Mission Vivekananda Educational and Research Institute, Narendrapur, Kolkata 700103, West Bengal, India.

View Book :- http://bp.bookpi.org/index.php/bpi/catalog/book/206