Bioremediation and Enzymatic Activities of Pseudomonas fluorescence and Candida parapsilosis| Chapter 6 | Innovations in Microbiology and Biotechnology Vol. 8

 Vegetable oil spills are appropriate more common and conceivably morechallenging than petroleum hydrocarbon spills. Microbial lipases are famous biocatalysts that are frequently used in the remediation of fat spills. For the remediation of locations adulterated with fat, a thorough description of lipase is required. This work was acted to monitor the degradation pattern of vegetable oil. The bioremediation experiment working two previously private microbial isolates from an oil mill in Ibadan, Nigeria. Soil samples from the Nursery portion of the Microbiology department, in addition to soil samples from the oil mill, were all endanger various treatment forms. Field bioremediation with the isolates was completed activity for 12 weeks, and lipase activity was more determined. Pseudomonas radiance and Candida parapsilosis were recognised as the two isolates. The result of sterile soil samples accompanying and without joining option, from touch oil and touch kernel intentionally contaminated soils for all the various situation,s showed a accepted increase in total viable counts from the 2nd period to the 12th week, nevertheless in thenon-sterilee counterpart skilled was a steady increase from the 2nd week to the 8th period and subsequently, a steady decrease from the 10th to the 12th week. Pseudomonas radiance and Candida parapsilosis were found to have the potential to shame fatty waste. As a result, they maybe used in the environmental explanation of the vegetable oil slop site.

Author(s) Details:

B. M. Popoola,
Department of Biological Sciences, Faculty of Natural Sciences, Ajayi Crowther University, Oyo, Nigeria.

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A Peep into Microbial Isolates from Various Vegetable Oil Polluted Sites | Chapter 11 | Recent Progress in Microbiology and Biotechnology Vol. 2

 Spills of vegetable oil are becoming popular and potentially more difficult than spills of petroleum hydrocarbons. Among biocatalysts, microbial lipases occupy a position of prominence, sometimes used for the remediation of contaminated vegetable oil sites. This work was carried out to isolate microorganisms and screen them for their lipolytic activity from oil-polluted sites. Microorganisms were extracted from eight experimental soil samples contaminated with various forms of vegetable oil, soil from the Ibadan oil mill, and usual uncontaminated soil as soil samples were also obtained from the Oyo town palm oil mill at the same time as the control. The isolates were characterised, categorised and their lipolytic activity was screened for those common to at least one of the experimental sites and oil mill sites. Using the Duncan Multiple Range Test, the data obtained was analysed. 73 microorganisms have been isolated from the contaminated soil and have been classified as Bacillus species (16), Pseudomonas (12), Micrococcus (1), Aspergillus (9), Penicillium (6), Saccharomyces (4), Geotrichum (1), Kluveromyces (1), Flavobacterium (6), Alcaligenes (2), Proteus (3). Bacillus subtilis, Bacillus licheniformis, Pseudomonas cepacia, Flavobacterium sp., Pseudomonas fluorescens, Alcaligenes sp. Aspergillus niger, Pseudomonas fluorescens, Bacillus subtilis, Bacillus licheniformis, Bacillus cereus, Bacillus circulans, Bacillus pumilus and Saccharomyces cerevisae were preliminarily screened for lipolytic activity and Candida parapsilosis, common to at least one of the experimental site and oil mill site in Ibadan, all nine verified by the existence of halos around the colonies The potential for oxidation of fatty waste is present in these tested species. Consequently, they may be used in the environmental clean-up of vegetable oil spill sites.

Author (s) Details

Bukola Margaret Popoola
Department of Biological Sciences, Ajayi Crowther University, Oyo State, Nigeria.

A. A. Onilude
Department of Biological Sciences, Ajayi Crowther University, Oyo State, Nigeria.

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