Studies on Eco Friendly Management of Mycoflora Associated with Trichosanthes anguina L. and Trichosanthes dioica Roxb. | Chapter 2 | Recent Research Advances in Biology Vol. 3

 Vegetables are plant components that are eaten as food by humans and as part of a savoury course or meal. Trichosanthes anguina L. is among all the vegetables. (Gourd of the snake) and Trichosanthes dioica Roxb. (Pointed gourd) is very common. An investigation into the control of fungi associated with two common Bangladeshi vegetables has been carried out. Anguina trichosanthes L. Uh, and T. Roxb Dioica. Throughout the duration from June to December, 2014. Fresh vegetables were apparently collected from five local markets in Dhaka City, near the Dhaka University campus. After harvest, within 3-4 days of short storage, the vegetables are infected with fungi. Fungi associated with the samples were extracted from the samples using the PDA (Potato Dextrose Agar) medium 'Tissue Planting' process. Seven fungal species, namely, Aspergillus flavus, A. A. Fumigatus. It was discovered that niger, Fusarium sp., Penicillium sp., Rhizopus stolonifer and Trichoderma viride were associated with the vegetables described above. The prevalent fungi associated with the snake gourd and the pointed gourd were Aspergillus niger. Present observations suggest that A. Niger is the primary fungus associated with each of the Karwan bazaar's collected vegetables. To regulate the extraction of fungi from three plants, namely Allium cepa, A. In order to evaluate their effect on radial growth, sativum and Zingiber officinale and two chemicals, namely sodium bicarbonate and sodium chloride, were selected for seven species of fungi isolated from fresh vegetables. Allium sativum extract and both chemicals demonstrated 100 percent radial growth inhibition at 20 percent concentrations of isolated fungi.

Author(s) Details

Abu-Al-Islam
Sonali Bank Ltd. Bangladesh and Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh.

Prof. Dr. Shamim Shamsi
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh.

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Mangrove Oysters: Mortality and Microbiota Population Dynamics during Processing and Ambient Storage | Chapter 15 | Recent Progress in Microbiology and Biotechnology Vol. 2

 

The molluscan bivalve shellfish Mangrove Oyster (Crassostrea gasar) is a typical inhabitant of the mangrove / estuarine ecosystem of Nigeria's Niger Delta. The dynamics of mortality and microbiota population of fresh, processed and stored mangrove oysters were investigated at ambient temperature. During purification in tap water (TW) and brackish water (BW) microenvironments, the mortality rates of raw oysters were calculated for 14 days. In the environment, mortality was observed on the 5th, 11th and subsequent days , respectively. The shelf-life of oysters was greatly improved in BW microcosms. It thus shows the beneficial effect of mangrove oyster depuration in BW as opposed to TW microcosms. Using standard microbiological methods, the microbial counts of raw (shucked), processed and oyster meat samples during ambient storage were determined. The aerobic plate counts (APCs) for raw (shucked) and processed oyster meat samples on day 0 were 1.36 ⁇ 105 and 3.00x103 CFU / g, respectively, but After storage, it increased from 0-0.8 to 102 CFU / g. In numbers and variety, bacteria were more prevalent than fungi. Bacillus, Pseudomonas, Vibrio, Proteus and Staphylococcus were the most commonly isolated microbes from raw (shucked), processed and preserved oyster meat samples, while others were Aspergillus, Penicillium and Fusarium. Bacillus (20,8 percent) and Pseudomonas (16,7 percent); Aspergillus (52,3 percent) and Penicillium (45,4 percent) were the most dominant genera during storage. Non-detectability of Escherichia coli and Acinetobacter species as well as bioburden reduction, however, underlines the criticality and necessity of sufficient pre-consumption heat treatment for oysters, as some of these organisms are not only potentially pathogenic, but also of public health significance. This study highlights the marketability of depurated mangrove oysters to boost health risks and severe post-harvest economic losses on or before 5 days and 24h for processed samples.

Author (s) Details

Lawrence O. Amadi
Department of Microbiology, Faculty of Science, Rivers State University, P.M.B. 5080, Nkpolu-Oroworukwo, Port Harcourt, Nigeria and Department of Microbiology, School of Applied Science, Ken Saro-Wiwa Polytechnic, P.M.B. 20, Bori, Rivers State, Nigeria.

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