Taqman-based Reverse Transcription and Real-time PCR for Diagnosis of Peste des Petits Ruminants (PPR) /Small Ruminant Morbilli (SRM) Virus from Clinically Affected Animal Samples Collected in Thrissur District, Kerala State and Molecular Epidemiology of Recovered Isolates |Chapter 9 | Innovations in Biological Science Vol. 4

 

Peste des petits ruminants (PPR) is caused by a virus of the family Paramyxoviridae, genus Morbillivirus and species Morbillivirus caprinae or small ruminant Morbilli virus. PPR also known as ovine rinderpest is an acute, highly contagious, World Organization for Animal Health (WOAH) notifiable and economically important transboundary viral disease affecting primarily sheep and goats associated with high morbidity and mortality. Camels and wild small ruminants can also be affected by PPRV.  PPR is considered as one of the main constraints in augmenting the productivity of small ruminants in developing countries and severely affects poor farmer’s economies. The disease is clinically manifested by pyrexia, occulo-nasal discharges, necrotizing and erosive stomatitis, gastroenteritis, diarrhea, bronchopneumonia and can be diagnosed from classic clinical signs, pathological lesions, and specific detection of virus antigen-surface proteins/antibodies/viral RNA in the clinical samples by various immune chromatographic/serological tests and molecular assays. The current diagnostic strategy for PPR used in network laboratories of AHD Kerala is mainly based on serological assays.  The present study aimed to standardize Taqman-based reverse transcription Real-Time PCR (RT PCR) protocols for the detection of PPR virus in samples like EDTA blood collected during viraemic/febrile phase and tissue samples after post-mortem from clinically affected animals- and to study the molecular epidemiology of isolates recovered targeting Fusion protein (F) gene.

 

 

Author(s) Details

Sunitha Karunakaran

Animal Disease Control Project, Thrissur, India and Veterinary Dispensary, Koorkanchery, Thrissur, India.

Beena. P. S

Omicsgen Life Sciences Limited, North Kalamassery, Kochi, Kerala, India.

Gopika Thattat Gopinathan

Department of Animal Husbandry, Clinical Laboratory, Animal Disease Control Project, Thrissur, Kerala, India.

Hareesh Sasidharan

Animal Disease Control Project, Thrissur, India and Veterinary Dispensary, Koorkanchery, Thrissur, India.

 

Please see the book here:- https://doi.org/10.9734/bpi/ibs/v4/11456F

Taqman-based Reverse Transcription and Real-time PCR for Diagnosis of Peste des Petits Ruminants (PPR) /Small Ruminant Morbilli (SRM) Virus from Clinically Affected Animal Samples Collected in Thrissur District, Kerala State and Molecular Epidemiology of Recovered Isolates | Chapter 9 | Innovations in Biological Science Vol. 4

Peste des petits ruminants (PPR) is caused by a virus of the family Paramyxoviridae, genus Morbillivirus and species Morbillivirus caprinae or small ruminant Morbilli virus. PPR also known as ovine rinderpest is an acute, highly contagious, World Organization for Animal Health (WOAH) notifiable and economically important transboundary viral disease affecting primarily sheep and goats associated with high morbidity and mortality. Camels and wild small ruminants can also be affected by PPRV.  PPR is considered as one of the main constraints in augmenting the productivity of small ruminants in developing countries and severely affects poor farmer’s economies. The disease is clinically manifested by pyrexia, occulo-nasal discharges, necrotizing and erosive stomatitis, gastroenteritis, diarrhea, bronchopneumonia and can be diagnosed from classic clinical signs, pathological lesions, and specific detection of virus antigen-surface proteins/antibodies/viral RNA in the clinical samples by various immune chromatographic/serological tests and molecular assays. The current diagnostic strategy for PPR used in network laboratories of AHD Kerala is mainly based on serological assays.  The present study aimed to standardize Taqman-based reverse transcription Real-Time PCR (RT PCR) protocols for the detection of PPR virus in samples like EDTA blood collected during viraemic/febrile phase and tissue samples after post-mortem from clinically affected animals- and to study the molecular epidemiology of isolates recovered targeting Fusion protein (F) gene.

Author(s) Details:

Sunitha Karunakaran,
Animal Disease Control Project, Thrissur, India and Veterinary Dispensary, Koorkanchery, Thrissur, India.

Beena. P. S
Omicsgen Life Sciences Limited, North Kalamassery, Kochi, Kerala, India.

Gopika Thattat Gopinathan
Department of Animal Husbandry, Clinical Laboratory, Animal Disease Control Project, Thrissur, Kerala, India.

Hareesh Sasidharan
Animal Disease Control Project, Thrissur, India and Veterinary Dispensary, Koorkanchery, Thrissur, India.

 

Please see the link here: https://stm.bookpi.org/IBS-V4/article/view/14398

WGS: An Innovative Approach for Management of Tuberculosis | Chapter 8 | Advancement and New Understanding in Medical Science Vol. 2

Whole genome sequencing (WGS) of Mycobacterium tuberculosis is becoming a promising tool for clinical applications for the diagnosis and management of tuberculosis in public health care. Since absence of a consensus and international standards in the analysis and validation of quality controlling of WGS technology, result may lack harmonization and comparability for diagnosis. In this review, the significance of WGS in diagnosis and surveillance is outlined.

Author(s) Details:

Chamila Priyangani Adikaram,
National Tuberculosis Reference Laboratory, Central Public Health Laboratories, Ministry of Health, Muscat, Oman.

Please see the link here: https://stm.bookpi.org/ANUMS-V2/article/view/13094

Possible Risk Assessment of Dementia Due to Air Pollution in Czech Republic| Chapter 2 | Current Overview on Disease and Health Research Vol. 9

 A review of current literature signifies a significant effect of air pollution on the raised incidence of senility, particularly Alzheimer’s disease. Dementia, encompassing two together vascular dementia and Alzheimer’s disease, is immediately reported as the leading cause of cessation in England and Wales, accounting for 12% of all recorded deaths. Ambient air contamination is a pervasive and ubiquitous hazard, that has been connected to premature morbidity and a increasing number of morbidity endpoints. Air dirtiness may be linked to neurodegeneration, and by way of this or other pathways, to neurodegenerative afflictions. Inflammation and oxidative damage are two potential mechanisms. The excavating regions of Northern Bohemia in the Czech Republic experienced intensely high air contamination levels in previous decades, and during ultimate recent ending, Northern Moravia also experienced this issue. By joining cutting-edge microscopic epidemiology methods with a thorough test of the lifestyle selections of the population residing in violated areas, this particular situation could be used to study the friendship between air contamination and dementia.

Author(s) Details:

J. Topinka,
Department of Genetic Toxicology and Epigenetics, Institute of Experimental Medicine AS CR, Videnska 8 1083, 142 20 Prague 4, Czech Republic.

P. Rossner,
Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine AS CR, 10 Videnska 1083, 142 20 Prague 4, Czech Republic.

A. Rossnerova,
Department of Genetic Toxicology and Epigenetics, Institute of Experimental Medicine AS CR, Videnska 8 1083, 142 20 Prague 4, Czech Republic.

R. J. Sram,
Department of Genetic Toxicology and Epigenetics, Institute of Experimental Medicine AS CR, Videnska 8 1083, 142 20 Prague 4, Czech Republic.

Please see the link here: https://stm.bookpi.org/CODHR-V9/article/view/9179

A Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter pylori Isolates from Southern Russia | Chapter 6 | Recent Progress in Microbiology and Biotechnology Vol. 7

 The genomes of Helicobacter pylori have a high genetic diversity and rapid microevolution, a characteristic that is thought to have a functional role in the persistence and colonisation of varied human populations. H. pylori isolates can be phylogeographically categorised. We compared MLVA genotypes of 48 H. pylori strains obtained from different locales in southern Russia to characterise diverse strains of this disease in different human populations. When compared to the four publicly accessible genomes, cluster analysis revealed the likely membership of the majority of the strains to the hpEurope population, and two strains to the hpEastAsia population. Every typical PCR laboratory has access to MLVA type, which is a faster and more uniform method for evaluating genetic relatedness. The proposed approach could be useful in molecular epidemiology and possibly for H. pylori population identification. For the first time, a similar study was done in Russia and Europe.

Author (S) Details

Vladimir Mihajlovich Sorokin
Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia.

Ruslan Vjacheslavovich Pisanov
Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia.

Elena Vadimovna Golubkina
Department of Biochemistry, Astrakhan State Medical University, Ministry of Health of Russia, Astrahan, Russia.

Elena Aleksandrovna Bereznyak
Laboratory of Biological Safety and Highly Dangerous Infections Treatment, Rostov-on-Don Research Anti-Plague Institute, Russia.

Lubov Aleksandrovna Prozorova
Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia.

View Book :- https://stm.bookpi.org/RPMB-V7/article/view/2576