Curing of Mammalian Cell Lines from Severe Bacterial Contamination: A Descriptive Approach | Chapter 10 | Innovations in Microbiology and Biotechnology Vol. 5

The use of mammalian cell culture in the study of human cellular physiological processes has been demonstrated. Microbial contamination, on the other hand, is still a prevalent and important problem for researchers around the world, and it happens even when strict aseptic procedures are followed. This can have major consequences, such as corrupted results and the loss of crucial biological samples due to erroneous, non-reproducible data. Mycoplasma is the most prevalent causal agent found in mammalian cell lines. The identification and eradication of bacterial contamination observed during mammalian cell line culture are described in this article. Because of the contamination, we were on the verge of losing the cell lines. Using 16S rDNA sequencing, the contaminant was identified as gram-positive bacteria from the species Bacillus that was already resistant to penicillin and streptomycin. The cultures were treated for two weeks with a cocktail of antibiotics, including ciprofloxacin and gentamicin, in varied doses to determine the most efficient curing dose with the least cytotoxicity. The cells were eventually found to be bacterial-free. As a result, even in the absence of additional antimicrobial treatment, cell lines maintained for an extended period of time (under the microscope) remained healthy and normal. Finally, using FACS, we discovered that these cells preserved their typical cell cycle features.

Author (s) Details

Ms. Garima Singh
Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi, UP- 221005, India.


Ms. Kumud Tiwari
Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi, UP- 221005, India.


Mr. Samarendra K. Singh
Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi, UP- 221005, India and School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi, UP- 221005, India.


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Study on Utilization of Prosopis juliflora Root Powder as a Self Curing Agent | Chapter 9 | New Visions in Science and Technology Vol. 4

Curing is a crucial step in increasing the strength of concrete. A self-curing agent's function is to conserve water and reduce water evaporation from concrete, hence increasing the water retention capacity of concrete when compared to conventionally cured concrete. In this study, Prosopis juliflora root powder is utilised as an additive for self-curing concrete. Due to its mechanism for overcoming harsh situations such as drought and salt, Prosopis juliflora (Seemai Karuvelam in Tamil) grows rapidly and spreads. It can draw water from deeper strata thanks to its deep penetrating roots. Because its root can hold a lot of water, powder formed from it can be used as an additive in self-curing concrete. The experimental analysis of the self-curing property of concrete using Prosopis juliflora root powder is the focus of this work. The tests were carried out on both fresh and hardened concrete. Based on the findings, we concluded that by including Prosopis juliflora root powder as a self-curing agent in the concrete mix at various percentages, the addition of 0.75 percent Prosopis juliflora root powder by the weight of cement achieves the 28-day compressive strength of conventional M25 grade concrete in just seven days. Concrete's compressive strength is reduced by adding more Prosopis juliflora root powder.

 

Author (S) Details

S. Karthikeyan
Department of Civil Engineering, Kongu Engineering College, Perundurai, Tamil Nadu, India.

V. Pradeesh
Department of Civil Engineering, Kongu Engineering College, Perundurai, Tamil Nadu, India.

D. Prabakaran
Department of Civil Engineering, Kongu Engineering College, Perundurai, Tamil Nadu, India.

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Experimental Investigation of Characterization of Isotropic Magnetorheological Elastomers| Chapter 8 | New Approaches in Engineering Research Vol. 2

 The magnetorheological elastomer is a new form of smart material (MRE). Its dynamic mechanical performances can be controlled by controlling an applied magnetic field. MRE is commonly utilised in semi-active vibration absorbers as a stiffness - adjustable spring. For a perfect vibration control effect, MRE must have a low dynamic damping. This study shows how to make isotropic MREs at room temperature and pressure using a unique method. In the absence of a magnetic field, a variety of MR elastomer samples were created using Sylard's184 silicone elastomer with un-annealed electrolytic iron power 500 mesh and 15-micron size. Their dynamic properties have been studied, including fractional change in resonance frequency and fractional change in. The effects of iron particles and the applied magnetic field were investigated. This study should also be relevant in the development and preparation of novel MR elastomers.

 
Author (s) Details

Dr. S. R. Kumbhar
Department of Automobile Engineering, Rajarambapu Institute of Technology, Rajaramnagar, Maharashtra, India.

Prof. Dr. Subhasis Maji
School of Engineering & Technology (SOET), IGNOU, New Delhi- 110068, India.

Prof. Dr. Bimlesh Kumar
Government Polytechnic, Purnea, and Govt. Poly. Araria, Bihar, India.

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