Platelet Concentrates Bacterial Contamination. Seven Years Retrospective Study in Rabat Blood Center of Morocco | Chapter 14 | New Visions in Medicine and Medical Science Vol. 6

 Background: Bacterial contamination of blood products is still the largest residual source of transfusion transmitted diseases. The clinical consequences of transfusing bacterially contaminated blood range from minimal or no reaction to fatal septic shock and death.

Objective: Data from the 2005 to 2012 archives at the Laboratory of Bacteriology Serology and Hygiene of ibn Sina University Hospital Center in Rabat, Morocco was used to realize a retrospective study with the aim of determining the prevalence of bacterial contamination of platelet concentrates produced by the Regional Center for Blood Transfusion of Rabat after the implementation of the bacterial quality control of the platelet concentrates.

Methods: 3898 platelet concentrates, which were obtained after disinfection of the sampling site and diversion of the first milliliters of the blood donation, were cultured to study bacterial contamination between 2005 and 2012. All obtained bacterial colonies are tested by the Gram staining and orientation tests such as catalase, coagulase, and oxidase, as well as the biochemical gallery for identification. This retrospective study was conducted over a period of six months using data from the archives of the Laboratory of Bacteriology Serology and Hygiene of Ibn Sina University Hospital Center in Rabat, Morocco.

Results: 0.44% was the prevalence of bacterial contamination in platelet concentrates found in this retrospective study. It also showed the presence of the following bacteria species: coagulase-negative Staphylococcus species and Bacillus sp each at 29.41%, Staphylococcus aureus, Streptocoque, E. coli and Enterobacter each were found at 5.9%. Other bacilli’s grams negative were present at 17.65%.

Conclusion: Even though the prevalence of bacterial blood contamination found in this retrospective study was low, blood transfusion in Morocco has a long way to go before reaching the standards set and commonly accepted in developed countries.

Author(s) Details:

Fatna EL Mehdaoui
Research Laboratory, High Institute of Nursing Professions and Health Techniques of Rabat, Rabat, Morocco and Laboratory of Biology and Health, Kenitra Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco.

Raouf Alami
Research Laboratory, High Institute of Nursing Professions and Health Techniques of Rabat, Rabat, Morocco.

Abdelmajid Soulaymani
Laboratory of Biology and Health, Kenitra Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco.

Karim Souly
Laboratory of Bacteriology Serology and Hygiene, Ibn Sina University Hospital Center, Rabat, Morocco.

Please see the link here: https://stm.bookpi.org/NVMMS-V6/article/view/14358

Curing of Mammalian Cell Lines from Severe Bacterial Contamination: A Descriptive Approach | Chapter 10 | Innovations in Microbiology and Biotechnology Vol. 5

The use of mammalian cell culture in the study of human cellular physiological processes has been demonstrated. Microbial contamination, on the other hand, is still a prevalent and important problem for researchers around the world, and it happens even when strict aseptic procedures are followed. This can have major consequences, such as corrupted results and the loss of crucial biological samples due to erroneous, non-reproducible data. Mycoplasma is the most prevalent causal agent found in mammalian cell lines. The identification and eradication of bacterial contamination observed during mammalian cell line culture are described in this article. Because of the contamination, we were on the verge of losing the cell lines. Using 16S rDNA sequencing, the contaminant was identified as gram-positive bacteria from the species Bacillus that was already resistant to penicillin and streptomycin. The cultures were treated for two weeks with a cocktail of antibiotics, including ciprofloxacin and gentamicin, in varied doses to determine the most efficient curing dose with the least cytotoxicity. The cells were eventually found to be bacterial-free. As a result, even in the absence of additional antimicrobial treatment, cell lines maintained for an extended period of time (under the microscope) remained healthy and normal. Finally, using FACS, we discovered that these cells preserved their typical cell cycle features.

Author (s) Details

Ms. Garima Singh
Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi, UP- 221005, India.


Ms. Kumud Tiwari
Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi, UP- 221005, India.


Mr. Samarendra K. Singh
Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi, UP- 221005, India and School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi, UP- 221005, India.


View Book :- https://stm.bookpi.org/IMB-V5/article/view/6571