Showing posts with label coliforms. Show all posts
Showing posts with label coliforms. Show all posts

Thursday, 20 April 2023

Reducing Bacterial Contamination of Fresh Cabbage Using Vinegar and Salt | Chapter 2 | Current Perspectives in Agriculture and Food Science Vol. 3

 Green vegetable has great pertaining to food and therapeutic value and is generally used as the vital element in salads. Unfortunately, they are prone to contamination ahead the value chain. This work proposed to evaluate the microorganisms population in different divisions of green cabbage heads got from five selected markets in Accra and also to decide the most appropriate means for reducing the microorganisms population before consumption. Samples were got from five open markets in the Greater Accra Region of Ghana. Fresh leaves taken from the samples' exposed, middle, and inner sections were resolved for aerobic mesophilic organisms, coliforms, Staphylococcus aureus, Bacillus cereus, and Escherichia coli. They were laundered with water from faucet, 5% salt solution, or 50% salt solution solution for 5 brief time period, and the effectiveness of the situations in reducing the bacteria society was determined. The study revealed that the outer portions of fresh green vegetable heads had the highest microbial load, trailed by the middle sections, and the central sections were almost sterile. Coliforms ranged from 104–106 CFU/g for the not cooked outer divisions, 102–104 CFU/g for the untreated middle portions, and 101 CFU/g for the untreated inner divisions. The treatment accompanying 50% vinegar solution was ultimate efficient in lowering the bacteria populace on the samples, followed by 5% salt resolution, and tap water was the slightest effective. The findings concerning this study can be used to raise knowledge about the limited influence of the use of ordinary tap water by caterers to wash cabbages and advance the use of vinegar or seasoning solutions to reduce microbial adulteration in cabbages.

Author(s) Details:

Amy Atter,
CSIR- Food Research Institute, Ghana.

Damian Amewowor,
University of Cape Coast, Ghana.

Wisdom Kofi Amoa-Awua,
CSIR- Food Research Institute, CSIR College of Science and Technology, Ghana.

Please see the link here: https://stm.bookpi.org/CPAFS-V3/article/view/10233

Monday, 4 January 2021

Molecular Diagnostic Techniques for the Characterisation of Escherichia coli | Chapter 11 | Recent Progress in Microbiology and Biotechnology Vol. 4

 Escherichia coli isolated from Creek Road/Bonny Estuary using molecular techniques was described by this research. For the presence of E, one hundred and twenty (120) water samples were examined. Coli at Bonny Estuary/Creek Lane. For the isolation and detection of E, the Most Probable Number (MPN) process, the Eijkman test and molecular techniques were used. From coli. The research was performed on a daily basis (morning and evening) and seasonally (end of rainy season (November), beginning of rainy season (April) and mid rainy season (July) respectively). All of E. In the E pathotypes, coli isolates were screened for resistance genes. The Polymerase chain reaction technique uses coli (EHEC, EPEC, EAEC, ETEC and EIEC), including Sulfhydryl Variable (SHV), CTX-M, Temoniera (TEM) and MCR genes. The findings showed that 100% of the water samples were positive for coliforms and that E was harbouring all the water samples. From coli. In the morning of April (48 MPN/100 ml), the highest total count of coliforms recorded was The distribution of in the E of individual genes. SHV (5.0), CTX-M (5.8), TEM (4.2), MCR (0.0 percent), stx1 and stx2 (4.2), esV and bfA (1.7), aaiC (4.2), elt (3.3), and invE are coli isolates: (2.5). Of all the resistance genes, CTX-M was the most frequently detected. The presence of bacteria in the water body may have led to the location of waste dumps, the discharge of sewage and the construction of toilets, and the use of human and animal excreta as manure.

Author(s) Details

Dr. Constancy Prisca Aleru
Department of Medical Laboratory Science, Rivers State University, Nkpolu-Oroworukwo, P.M.B. 5080, Port Harcourt, Rivers State, Nigeria.

Prof. Kinikanwo Confidence Wachukwu

Department of Medical Laboratory Science, Rivers State University, Nkpolu-Oroworukwo, P.M.B. 5080, Port Harcourt, Rivers State, Nigeria.

View Book :-
https://bp.bookpi.org/index.php/bpi/catalog/book/359