Development and Validation of a HPLC Method for the Simultaneous Estimation of Simvastatin and Metformin | Chapter 10 | Advanced Concepts in Pharmaceutical Research Vol. 3

 Simvastatin is individual of the well-known statins that is a HMG-COA reductase inhibitor. Metformin hydrochloride is a well- known spoken antihyperglycemic drug. The present work has created and validated a precise and correct reverse phase liquid chromatographic means for the simultaneous estimation of simvastatin and metformin. This is cause the reversed step HPLC method is ultimate suitable for assessing two together ingredients happening at about the same time. The method was created utilizing a Jasco Auto polltaker HPLC with 50mM Sodium Dihydrogen Phosphate Monohydrate at pH 3.0 and Acetonitrile (30:70 v/v). UV-visible detectors were secondhand for the analysis, and 235 nm was used to monitor the eluents. Simvastatin and metformin were proved to have retention opportunities of 7.650 and 2.858 minutes, respectively. The approach was corroborated in terms of time, robustness, precision, and veracity. The planned method for the simultaneous quantitative perseverance was implemented efficiently.

Author(s) Details:

R. Varade,
Pacific University, Udaipur, Rajasthan, 313024, India.

H. Mishra,
K. J. Somaiya Institute of Engineering and Information Technology, Mumbai, MH, 400022, India.

Please see the link here: https://stm.bookpi.org/ACPR-V3/article/view/12703

Estimation of Metformin Hydrochloride, Gliclazide and Pioglitazone Hydrochloride: A UDDD-HPTLC Densitometry Method Validation Study | Chapter 12 | Novel Aspects on Pharmaceutical Research Vol. 3

 A plain and new UDDD-HPTLC method has happened developed and justified for their estimation of MET, GLZ and PIO all-inclusive and combined dose dosage form. Metformin (MET) is chemically, 1-carbamimidamido-N-N-dimethyl- methanimidamide. It is an oral antagonistic-diabetic drug from the biguanide class. It is the first-line drug for the treatment of type-2 diabetes, specifically in overweight and corpulent people and those accompanying normal sort function and evidences suggest possibly the best choice for people as political whole with heart attack.The  chromatographic  separation  of  these  drugs  was  completed activity  on  precoated  TLC  plates  silica  gel  60F254by  two  mobile steps consisting of Ammonium Sulphate: Methanol: Acetonitrile: Water (4:3:2:1) for MET and PIO and Toluene: Ethyl Acetate: Formic Acid (6:4:0.5) for GLZ individually for ideal separation and good judgment. The densitometric detection and calculation were carried out at 237 nm for MET and 200 nm for GLZ and PIO. The confirmation parameters were rigidly followed as per the ICH directions.Chromatographic separation of the standard answer of MET, GLZ and was performed. Briefly, the spot of the standard answer was applied on TLC plates. The TLC plates were developed by uninterrupted ascending growth by using miscellaneous solvents in the way that acetone, benzene, chloroform, ethyl acetate, intoxicating and toluene. The linearity range was got at 3000-8000ng/spot, 360-960 ng/spot, 90-240 ng/spot for MET, GLZ and PIO with r2value>0.999. The different parameters to a degree precision, reproducibility, strength were efficiently obtained believable. The proposed arrangement was successfully used for simultaneous perseverance of MET, GLZ and PIO in the commercial expression.In simultaneous belief, the different opposition of drugs makes it more burdensome to develop and confirm any chromatographic method. In comparison to HPLC, the submitted UDDD-HPTLC method is novel, less high-priced, simpler, smart, and more flexible. This method can be secondhand for routine quality control study because it authorizes simultaneous guess of all API on a single TLC plate accompanying a single application.

Author(s) Details:

Rajesh Varade,
Pacific University, Udaipur, Rajasthan-313024, India.

Harsha Mishra,
Pacific University, Udaipur, Rajasthan-313024, India and K. J. Somaiya Institute of Engineering and Information Technology, Mumbai, Mh-400022, India.

Please see the link here: https://stm.bookpi.org/NAPR-V3/article/view/10887

Study on Functional Outcome, Complication and Readmission Rate in Bilateral Simultaneous Versus Staggered Versus Staged Total Knee Replacement | Chapter 2 | Highlights on Medicine and Medical Science Vol. 13

 Background: The decision to perform bilateral total knee arthroplasty simultaneously, staggered, or staged is a hot topic in orthopaedic practises (BTKA). There are numerous research that compare the functional outcomes of bilateral simultaneous vs phased TKA. However, no studies have examined the functional outcome, concomitant complications, and 90-day readmission risk of staggered BTKA (done in a single hospital visit).

A retrospective evaluation of 300 TKA patients who had bilateral TKA was conducted. The patients were separated into three groups, each containing 100 patients in a staggered, phased fashion. We examine the complication and readmission rates in all three groups using a hospital electronic health records database. The Knee Society Score (KSS), Western Ontario and McMaster Universities Arthritis Index (WOMAC), Oxford Knee Score (OKS), Kujala score, and range of motion were used to assess functional outcomes prior to surgery (ROM).

The study included 234 females and 66 males, with the majority of females in a contemporaneous group and the majority of males in a staggered group. In our analysis, the average age group was 62.25 percent. The staged group had a considerably higher ASA score of 3. The patients in the staggered group had a higher KSS and Kujala score, as well as a better range of motion and a lower rate of readmission and complications. Simultaneous BTKA resulted in a slightly greater rate of complications.

Conclusion: Although there is no significant difference in functional outcome between simultaneous, staged, and staggered bilateral TKA, the staggered group still has a better postoperative functional outcome.

Author (S) Details

Vishal Pusshkarna
Gujarat Adani Institute of Medical Sciences, India.

A. V. Gurava Reddy
Sunshine Hospitals, India.

View Book :- https://stm.bookpi.org/HMMS-V13/article/view/2721