Impact of Methyl Parathion on Proteins and Esterases Patterns in Brain Tissue of Fresh Water Cat Fish Heteropneustes fossilis (Bloch) through Polyacrylamide Gel Electrophoresis | chapter 6 | Contemporary Research and Perspectives in Biological Science Vol. 2

The present investigation aimed to assess the impact of Methyl Parathion, an organophosphate compound, on the protein and esterase isozyme banding patterns within the brain tissue of the freshwater catfish Heteropneustes fossilis (Bloch) over various time intervals (24, 48, 72, and 96 hours), in comparison to a control group. The analysis of protein banding patterns was conducted utilizing a standard marker, and Rm values were calculated as part of the study. The results indicated that exposure to methyl parathion induced significant alterations in the protein patterns across various tissues, including the liver, muscle, gut, and brain of Heteropneustes fossilis, leading to similarities in protein bands with minor differences. All three esterase bands were identified in the brain tissue. In liver tissue, Est-1 was found to be the most prominent at 24 hours, while Est-2 and Est-4 were most intense at 72 hours. Additionally, Est-2 and Est-3 exhibited weak staining in brain tissue, with Est-3 also showing weak staining in liver tissue and Est-4 in gut tissues. The results further illustrated that exposure to methyl parathion resulted in notable changes in the esterase patterns of various tissues, including the brain tissue of H. fossilis, which displayed similarities in protein bands with slight variations.

 

Author (s) Details

T. Bheem Rao
Department of Zoology, Kakatiya Government College, Hanamkonda, India.

 

Please see the book here:- https://doi.org/10.9734/bpi/crpbs/v2/248

Exploring the Impact of Sodium Arsenite on Lipid Metabolism in Heteropneustes fossilis and the Potential Chelating Effect of Zeolite | Chapter 8 | Innovations in Biological Science Vol. 3

Arsenic toxicity is a global health problem affecting many millions of people. Arsenic, the metal pollutant found naturally in groundwater and unnaturally in mine waste sites and agricultural runoff, has been considered toxic to humans for several millennia. In the present work the effect of different concentrations of sodium arsenite on lipid metabolism in Heteropneustes fossilis, and the chelating effect of synthetic zeolite was studied from liver.
 
Fishes were exposed to two different concentrations of sodium arsenite (200 ml and 400 ml of 1% solution), for 3 different durations (3days, 7 days and 15 days). The concentrations of total Cholesterol (F=6.35>3.84 at 5% P), HDL (F=14.91>6.83 at 5% P), LDL (F=10.11>1.39at 5% P) and triglyceride (F=42.1>19.34 at 5% P) was found significantly increased along with increasing concentration and duration of sodium arsenite. The toxic effect was found recovered after the application of synthetic zeolite for all parameters, i.e., total cholesterol (F=8.04>3.12 at 5%P), HDL (F=14.96>6.83 at 5%P), LDL (F=1.57>1.39 at 5% P) and triglyceride (F=26.28>19.34 at 5% P). Statistical analysis of the results was done by two-way ANOVA.
 
The results suggest that zeolite is a potential compound for decreasing significantly the load of toxicity of arsenic in aquatic fauna.

Author(s) Details:

Jayasree Balasubramanian,
Department of Zoology, St. Thomas College, Bhilai, CG, India.

Anil Kumar,
Department of Zoology, Govt.V.Y.T.PG. Autonomous College, Durg, CG, India.

Please see the link here: https://stm.bookpi.org/IBS-V3/article/view/14248