Analyzing the Genotoxic Effect of Heavy Metals Cr, Cu, Pb and Zn Using Allium cepa L. | Chapter 4 | Current Topics on Chemistry and Biochemistry Vol. 9

 This unit aims to analyze the cytotoxic belongings of chromium, copper, lead and metallic mineral in A. cepa root tip squash mitotic container estrangements.  Angiosperms are employed in listening studies because they are acknowledged as suitable hereditary models for detecting heavy metal-located environmental mutagens. Allium cepa (onion) has happened used to assess DNA damage in the way that chromosome aberrations and mitotic era irregularities. The root tips were treated accompanying three concentrations, viz. 5, 10 and 20 mg/100 ml of chromium, policeman, lead and zinc at range temperature for 24 h. Mitotic indications and chromosomal abnormalities were computed. These weighty metals were found to cause chromosomal deformities such as  Chromosome break, Chromosome bridge, C-formation of cells by dividing, Vagrant, Delayed Anaphase and Vagrant, Chromosome Loss, Polyploidy and Chromosome Bridge, Chromosome Loss and Loculated Nucles, Stickiness, Multipolarity and Polyploid prophase in addition to the increasing doses. Chromium and lead were raise to be more poisonous than copper and metallic mineral to the root meristem of A. cepa at a concentration of 20 mg/100 ml. The listing of cytotoxic potentials was in the descending order: lead > chromium > policeman > zinc.

Author(s) Details:

C. Sathya,
PG & Research Department of Biotechnology, National College, Tiruchirappalli 620 001, Tamil Nadu, India.

M. N. Abubacker,
Department of Botany, Bishop Heber College, Tiruchirapalli 620 017, Tamil Nadu, India.

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Cytogenetic Abnormalities Found in Patients with Infertility and Recurrent Spontaneous Abortions | Chapter 17 | Highlights on Medicine and Medical Science Vol. 14

 The main goal of this study was to find out how often chromosomal aberration is in infertile couples and what forms of chromosomal aberration they have. Infertility and first-trimester pregnancy loss are common problems for many couples, and the major reason they look into it is their chromosomes. Due to infertility or a higher incidence of spontaneous abortions, 266 couples visited the Center for Genetics of the Medical Faculty-University of Sarajevo for chromosomal cytogenetic examination. Each couple was given a karyotype using the GTG procedure and lymphocyte cultures from peripheral blood. In total, 15 out of 266 couples had chromosomal abnormalities (9 percent ). Couples with recurrent spontaneous abortions (107), and couples with infertility (159), were classified by primary diagnoses. Four males and one female were discovered to have chromosomal alterations in the first group, while seven females and three males were found to have chromosomal changes in the second. The major cause of infertility and recurring spontaneous abortions was structural and numerical chromosomal alterations, which did not differ from relevant updated references from other nations. The incidence and distribution of chromosomal abnormalities in couples with recurrent miscarriage and infertility are depicted in this study. Before deciding on further reproductive options, couples with an atypical karyotype should undergo genetic testing and counselling.

Author (S) Details

Mirela Mackic-Durovic
Center for Genetic, Faculty of Medicine, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.

Dunja Rukavina
Department of Biology, Veterinary Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.

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