Showing posts with label Acrisols. Show all posts
Showing posts with label Acrisols. Show all posts

Saturday, 16 September 2023

Characteristics of Rhizosphere Bacteria of Sugarcane (Saccharum spp.) Grown on Acrisol and Ferrasol Soils of Dong Nai Province, the Southeast of Vietnam | Chapter 8 | Emerging Issues in Agricultural Sciences Vol. 7

 The study proposed to investigate variety, population dynamics of nitrogen obsession and phosphate solubilization bacteria in rhizosphere of sugarcane refined in acrisol and ferrasol soils of Dong Nai province, the Southeast of Vietnam. Nitrogen total, dissolved phosphorus and basic carbon of the soil were determined by utilizing colorimetric method and design described by Walkley-Black. Nitrogen obsession and phosphate solubilization bacteria were isolated on Burk's nitrogen free and NBRIP publishing. Identification, SNPs and nucleotide diversity of picked bacterial strains were based on 16S rDNA sequences accompanying primers 27F and 1492R, SeqScape@Software and Theta (θ) described by Halushka et al.. Validation of nitrogen obsession of strains was carried out by hide presence of nifH accompanying Pol primers. The research found that rhizosphere of sugarcane refined in Dong Nai province were slightly sour, poorly nutrient and had different plant growth advancing bacteria. pH, nitrogen total and organic matter component of the soils were three essential determinants influencing two together populations of nitrogen obsession and phosphate solubilization bacteria in the soils while available planet seen at dawn in the soils was closely accompanying with nitrogen obsession bacteria society rather than phosphate solubilization bacteria populace in the soils. Thirty-one strains unique expressed volume of fixing nitrogen, solubilizing phosphate and biosynthesizing IAA. Fourteen of thirty-one strains were deepened nifH. Twelve of thirty-one strains accompanying high ability of plant growth publicity were identified as species of Bacilli, Acidobacteria, Bacteroides and Betaproteobacteria place Bacilli had the highest Pis and thetas. Bacillus megaterium B6 unique from ferrasols and Sphingomonas sp. P14 isolated from acrisols displayed as potential candidates and were proposed expected tested their skill to promote plant tumor of sugarcane grown in acrisol and ferrasol soils of Dong Nai province.

Author(s) Details:

Hoang Minh Tam,
Faculty of Natural Science Pedagogy, Sai Gon University, HCM City, Vietnam.

Cao Ngoc Diep,
Institute of Food and Biotechnology, Can Tho University, Can Tho City, Vietnam.

Please see the link here: https://stm.bookpi.org/EIAS-V7/article/view/11853

Wednesday, 1 December 2021

Study on Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea mays L.) Cultivated on Acrisols of the Southeast of Vietnam | Chapter 6 | Innovations in Microbiology and Biotechnology Vol. 2

 The variety of endophytic bacteria in maize (Zea mays L.) grown on Acrisols in Southeast Vietnam was investigated. In this region, twenty-eight maize stem and root samples were gathered from five different locations (provinces/cities). Endophytic bacteria were isolated in two mediums (LGI and NFb) and 16S rRNA gene fragments were amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 301 isolates were isolated, and all of them were capable of nitrogen fixation, phosphate solubilization, and IAA biosynthesis, but 30 isolates stood out, and they were recognised as maize endophytes and nifH gene owners. The genomes of 30 endophytic bacteria isolates were found to have a high degree of similarity to the GenBank reference strains (between 97 percent and 100 percent ). Six isolates belonged to Bacillus (20.00%), and 24 isolates belonged to Proteobacteria (80.00%), including two alpha-proteobacteria (7%), six beta-proteobacteria (20%), and sixteen gamma-proteobacteria (16 gamma-proteobacteria (16 gamma-proteobacteria (16 gamma-proteobacteria (16 gamma-p (53 percent ). Proteobacteria had the highest Theta values based on Pi value (nucleotide diversity), and Theta values (per sequence) from S of SNP for DNA polymorphism were computed for each group, with Proteobacteria having the highest values in comparison to two groups. Because of their usefulness and biosafety, three isolates, DTN1b (Azotobacter vinelandii), VTN2b (Bacillus subtilis), and VTN7 (Enterobacter cloacae), have been recommended as suitable microbial inoculants or biofertilizers for sustainable maize cultivation in low Acrisols in Vietnam.


Author(S) Details

Dang Thi Ngoc Thanh
Department of Natural Science Teacher Training, Sai Gon University, HCM City, Vietnam.

Cao Ngoc Diep
Department of Microbiology Biotechnology, Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam.

View Book:- https://stm.bookpi.org/IMB-V2/article/view/4718