Assessment of Morphological and Biochemical Characterization of Endophytic Bacteria from Leaves of Tamarind (Tamarindus indica) | Chapter 11 |Innovations in Microbiology and Biotechnology Vol.4

Endophytic bacteria were isolated from the leaves of Tamarindus indica and their morphological characterization was performed in this work. Endophytes are organisms that reside within the host plant without creating any visible disease symptoms. The goal of the research was to determine the various morphological properties of endophytic bacteria found in Tamarindus indica leaves. Various morphological features of isolated endophytic bacteria from Tamarindus indica leaves were used to make a preliminary identification of the endophytic bacterial isolates. The growth features of endophytic bacteria isolated from Tamarindus indica on King's B media revealed that colonies were either irregular or circular in shape, had a flat or elevated elevation on the petri plate, had an undulated or whole edge, and had an opaque and white surface. All of the endophytic bacteria isolated from Tamarindus indica leaves were non-haemolytic. Isolated endophytic bacteria from Tamarindus indica grew in BHI broth with turbidity and pellicle production, which could be sediment or rings. The microscopic examination of endophytic bacterial isolates revealed that all Tamarindus indica endophytic bacterial isolates were gramme positive rods. Endophytic bacteria isolates were also subjected to a battery of biochemical tests. Endophytic bacteria were identified based on the data, offering insight into the nature of the microbiome found in Tamarind leaves (Tamarindus indica).

Highlights
Endophytic bacteria isolated from Tamarindus indica leaves had circular colony features with wavy mucoid and a soft uneven border.

The colonies were complete, and the growth's surface was smooth, opaque, and white in colour.

Author(s) Details:

Alka Sawarkar,
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur (M.P), India and Department of Veterinary Pharmacology and Toxicology, Nagpur Veterinary College, MAFSU, Nagpur, (Maharashtra), India.


R. K. Sharma,
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur (M.P), India.


Vidhi Gautam,
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur (M.P), India.


Madhu Swamy,
Department of Veterinary Pharmacology and Toxicology, Nagpur Veterinary College, MAFSU, Nagpur, (Maharashtra), India.


Anju Nayak,
Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur (M.P), India.


Kabita Roy,
Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, NDVSU, Jabalpur (M.P), India.

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Study on Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea mays L.) Cultivated on Acrisols of the Southeast of Vietnam | Chapter 6 | Innovations in Microbiology and Biotechnology Vol. 2

 The variety of endophytic bacteria in maize (Zea mays L.) grown on Acrisols in Southeast Vietnam was investigated. In this region, twenty-eight maize stem and root samples were gathered from five different locations (provinces/cities). Endophytic bacteria were isolated in two mediums (LGI and NFb) and 16S rRNA gene fragments were amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 301 isolates were isolated, and all of them were capable of nitrogen fixation, phosphate solubilization, and IAA biosynthesis, but 30 isolates stood out, and they were recognised as maize endophytes and nifH gene owners. The genomes of 30 endophytic bacteria isolates were found to have a high degree of similarity to the GenBank reference strains (between 97 percent and 100 percent ). Six isolates belonged to Bacillus (20.00%), and 24 isolates belonged to Proteobacteria (80.00%), including two alpha-proteobacteria (7%), six beta-proteobacteria (20%), and sixteen gamma-proteobacteria (16 gamma-proteobacteria (16 gamma-proteobacteria (16 gamma-proteobacteria (16 gamma-p (53 percent ). Proteobacteria had the highest Theta values based on Pi value (nucleotide diversity), and Theta values (per sequence) from S of SNP for DNA polymorphism were computed for each group, with Proteobacteria having the highest values in comparison to two groups. Because of their usefulness and biosafety, three isolates, DTN1b (Azotobacter vinelandii), VTN2b (Bacillus subtilis), and VTN7 (Enterobacter cloacae), have been recommended as suitable microbial inoculants or biofertilizers for sustainable maize cultivation in low Acrisols in Vietnam.

Author(S) Details

Dang Thi Ngoc Thanh
Department of Natural Science Teacher Training, Sai Gon University, HCM City, Vietnam.

Cao Ngoc Diep
Department of Microbiology Biotechnology, Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam.

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