Production, Physical Optimization, and Purification of Xylanase Enzyme from Bacillus sp. by Solid State Fermentation | Chapter 10 | Microbiology and Biotechnology Research: An Overview Vol. 1

Aim: Xylanases are extensively applied in paper and pulp industries as well as during the preparation of baked products to improve their quality. Additionally, it is also used in coffee, oil, and starch industries in order to increase their nutritional values.

Place and Duration of the Study: Azyme Biosciences Laboratory, Bangalore. An experiment was conducted between January 2021 and June 2021.

Methodology: In the present study, soil samples were collected near sawmills in various localities of urban Bangalore to isolate organisms for the production of xylanase using solid-state fermentation. Six organisms were isolated using selective media based on their morphological characters. Among them, one organism showed maximum production of xylanase enzyme identified as Bacillus sp based on their biochemical test and 16s RNA sequencing. Solid substrate fermentation was carried out using various agro wastes such as sugar cane bagasse; sawdust, paddy husk, wheat straw, and orange peel powder.

Results: In the results, production revealed that sugarcane bagasse showed maximum production of enzyme Bacillus sp compared to other substrates with different physical parameters such as pH 8, and temperature at 35 ^ºC after 72 hrs incubation. Trace element such as Mg⁺⁺ enhances the production of enzymes by more than 22% compared with other metal ions like Ca⁺⁺, Mn⁺⁺ and Fe⁺⁺. After production, the enzyme is purified by using three-step methods ammonium sulphate precipitation, dialysis, ion exchange, and gel filtration. Fold purification was increased up to 12 fold, yielding 36% and the molecular weight of the enzyme was 62 KDa determined using SDS PAGE.

Conclusion: Bacillus sp shown in this experiment is capable of producing xylanase enzyme when subjected to different physical parameters.

 

Author (s) Details

Shylesha, B.S.
Department of Chemistry, Sree Siddaganga College of Arts, Science and Commerce, Tumakuru-572102, Karnataka, India.

 

Mahesh, M.
Department of Biotechnology, Azyme Biosciences Private Limited, Bengaluru-560069, Karnataka, India.

 

Shubharani, R
Department of Biotechnology, Azyme Biosciences Private Limited, Bengaluru-560069, Karnataka, India.

 

V.N. Yoganandamurthy
Department of Biotechnology, Azyme Biosciences Private Limited, Bengaluru-560069, Karnataka, India.

 

Please see the book here:- https://doi.org/10.9734/bpi/mbrao/v1/4021