The objective of the present study was to evaluate the in
vitro antioxidant and antibacterial activities of methanol extracts of
Catharanthus roseus and Ocimum basilicum. Plants are a rich source of
therapeutic compounds that have tremendous applications in the pharmaceutical
industry. Medicinal plants have been used to treat human diseases for thousands
of years because they have a vast and diverse assortment of organic compounds
that can produce a definite physiological action on the human body. The methanol
extracts of the leaves were used to investigate the antioxidant activity using
DPPH (2,2-Diphenyl-1-picrylhydrazyl) and superoxide radical scavenging assays.
The disc diffusion method was employed to evaluate the antibacterial activities
of four major infective pathogenic agents, namely, Escherichia coli,
Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis.
Phytochemical screening of the extract shows that the plant leaves are rich in
flavonoids, alkaloids, glycosides, saponins, steroids, terpenoids, tannins and
phenolic compounds. Both the plant extracts exhibited strong DPPH and
superoxide radical scavenging activities in a dose-dependent manner. The DPPH
scavenging activities by Catharanthus roseus and Ocimum basilicum extracts were
found to be 78.62±5.4% and 62.72± 4.2%, respectively. At the concentration of
100 µg/ml, the superoxide radical-scavenging activities of methanol extracts of
Catharanthus roseus and Ocimum basilicum were found to be 72.36±3.21 and
68.76±3.16, respectively. Moreover, both the extracts showed a remarkable
inhibition of bacterial growth at a concentration of 300 μg/ml compared to the
two other doses tested (100 and 200 μg/ml). The overall results of this study
can be considered as very promising in the perspective of obtaining new drugs
from plant sources, especially when the medical importance of the tested
microorganisms is considered. The current research offers experimental proof
that the leaves of Ocimum basilicum and Catharanthus roseus possess potential antibacterial
and antioxidant properties, suggesting that they could be employed as a safe
treatment for infectious diseases. Further studies are warranted to elucidate
and understand the antioxidants and antibacterial constituents / active
principles of these plants using various biochemical and molecular biology
tools such as LC/MS and GC/MS and in vivo studies.
Author(s)details:-
Rajamanickam Karthika
Department of Biochemistry, Mohamed Sathak College of Arts and Science,
Affiliated to University of Madras, Chennai - 600 119, Tamil Nadu, India.
Packirisamy Meenatchi
Department of Biochemistry, Mohamed Sathak College of Arts and Science,
Affiliated to University of Madras, Chennai - 600 119, Tamil Nadu, India and
Department of Biochemistry, Bharathidasan University Constituent College for
Women, Orathanadu 614 625, Thanjavur-District, Tamil Nadu, India.
Ramalingam Sundaram
Central Research Laboratory, Meenakshi Ammal Dental College and Hospital,
Maduravoyal, Tamil Nadu, India.
Ayyakkannu
Purushothaman
Department of Biochemistry, Mohamed Sathak College of Arts and Science,
Affiliated to University of Madras, Chennai - 600 119, Tamil Nadu, India and
Department of Biochemistry & Microbiology, PERI College of Arts and Science
(Affiliated to University of Madras), PERI Knowledge Park, Chennai - 600 048,
Tamil Nadu, India.
Please See the book
here :- https://doi.org/10.9734/bpi/ibs/v5/213
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