The present study primarily focuses on the determination of
emitted hydrogen
(H2) from bacterial cultures in closed septum
vials and both techniques, static-GC
as well as the sensor approach. When microorganisms are
cultivated in suitable
nutrient medium in closed septum vials, their emission of
hydrogen (H2) is
detected using a particular hydrogen sensor in addition to
headspace-gas
chromatography. Facultative anaerobes are able to release
both CO2 and H2
which are important chemicals for both types of bacteria,
whereas obligatory
aerobic bacteria can only create CO2 and water through
aerobic oxidation.
Obligate anaerobic bacteria emit H2 too but need an
oxygen-free atmosphere
which can be achieved if the air in the septum vial is
replaced by nitrogen. The
samples under investigation, either solid or liquid samples
and from smears by
wads from a cotton bud, are cultured in the headspace vials
and the hydrogen
emission was monitored after the necessary time of
incubation and thus microbe
contamination was detected. Antibiotics added to the
bacterial culture in the vial
are found to be effective if any gas emission is suppressed.
If not, they are either
ineffective or the bacteria are even resistant. Antibiotics,
both synthetic and
natural, were investigated and some were discovered to be
resistant or
ineffective. This technique was used to look into food
contamination by bacteria,
household necessities, medical specimens, and Lyme
borreliosis caused by tick
infection, as well as to diagnose and treat the disease. The
borrelia bacteria that
cause infection are found in ticks and, later on, in human
blood after they enter
the host. The foregoing examples show how essential oils can
stop bacterial
infections in various samples but this method can also be
applied in human
medicine and tick-borne Lyme disease is taken here as
representative for
diagnosis and therapy of diseases caused by bacterial
infections in humans. The
effect of the antibiotics applied can be examined and the
progress of an antibiotic
therapy can be controlled until its final success is
recognized.
Author (s) Details
Dr. Bruno Kolb
Student Research Centre, Überlingen, Obertorstrasse, Germany
Please see the link:-
https://doi.org/10.9734/bpi/rpmab/v3/370
No comments:
Post a Comment