Computational Approaches for Prediction of Conformational Epitopes for Birch Betv 1 and Hazel Cor A1 Targeting B-cells | Chapter 3 | Research Perspectives of Microbiology and Biotechnology Vol. 3

 

Through targeted interactions mediated by receptors on antibodies, B-cells

effectively neutralize invading pathogens by binding with remarkable precision to

specific molecular regions, called epitopes. This study focuses on enhancing B

cell epitope predictions via computational methods. Antigen sequences are

fragmented into peptides and docked against IgE antibodies to identify top

scoring interactions. Peptides with the highest scores undergo further analysis to

assess bond interactions. By examining overlapping sequences within the

antigen model, the positions of predicted epitopes are determined. Residues

involved in bond interactions are documented for these overlapping peptide

sequences. Validation is achieved through antigen-antibody docking studies to

confirm the predicted epitope sites. Ultimately, the integration of computational

and experimental methodologies holds promise for the rational design of

immunotherapeutic interventions targeting Birch and Hazel allergens, paving the

way for precision medicine in allergy treatment. 

 

Author (s) Details

Praveen Kumar Vemuri
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Green Fields, Vaddeswaram, Andhra Pradesh, India.

 

Varshitha Katta
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Green Fields, Vaddeswaram, Andhra Pradesh, India.

Anupama Ammulu Manne
Department of Civil Engineering, PVP Siddhartha Institute of Technology, Kanuru, Vijayawada, Andhra Pradesh, India.

Kanaka Durga Devi Nelluri
KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, Andhra Pradesh, India.

 Suryanarayana Veeravilli

Department of Humanities and Basic Sciences, Aditya University, Surampalem, Andhra Pradesh, India.

 KRS Sambasiva Rao

Department of Pharmacy, Mangalayatan University, Jabalpur, Madhya Pradesh, India.

Please see the link :-  https://doi.org/10.9734/bpi/rpmab/v3/187