Spirometra is a zoonotic parasite. An egg is the first stage in the life cycle of
Spirometra species. It can be used in the identification of
Spirometra species.
Spirometra eggs were collected from the faeces of
experimentally infected cats.
The eggs collected were used for morphological studies using
light microscope
and scanning electron microscope (SEM). Eggs collected from
faeces of cats
were fixed in 4% Glutaraldehyde, then post fixed in 1%
Osmium tetroxide,
dehydrated in ethanol series. Dehydrated material was dried
to a critical point
with carbon dioxide in a Polaron Critical Point Dry (CPD
7501). The dried
material was mounted on aluminium stubs. The specimen was
gold-coated in a
sputter coater (B10-RAD). Then eggs were examined in a
Philips SEM 515.
Scanning electron microscopy (SEM) images revealed the
presence of
operculum and operculum suture at one end of the egg.
Author(s) Details
Nicholas Jairo Kavana
Department of Microbiology and Parasitology, Faculty of
Medicine, St. Francis University College of
Health and Allied Sciences, Ifakara, Tanzania.
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