Yeast as a Versatile System for the Expression of Heterologous Proteins | Chapter 5 | Innovations in Microbiology and Biotechnology Vol. 1

 Because of its simplicity of genetic manipulation, faster generation time, availability of integrative and episomal vectors, and promoters that can be simply turned on and off using simple chemical moieties, the yeast Saccharomyces cerevisiae has been widely employed for heterologous protein production. Furthermore, as the simplest eukaryote, it has various benefits in the expression of mammalian proteins, such as post-translational modifications and the expression of membrane-bound proteins. The development of a strain for the production of membrane bound human drug metabolism enzymes is described in this article.

Author (S) Details

Rajeev Soni
Technical Operations, Genei Laboratories (a Rivaara Company), Bangalore, India.

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Comprehensive Proteomic Analysis of Lipid Rafts from RBL-2H3 Mast Cells| Chapter 9 | Recent Research Advances in Biology Vol. 9

 Lipid rafts are membrane microdomains rich in cholesterol, glycosphingolipids, and certain proteins that are highly organised. They play a role in biological activities in a variety of cell types, including mast cells (MCs). The protein composition of the MC lipid raft was determined utilising qualitative mass spectrometric analysis of the proteome extracted from detergent-resistant membrane fractions from RBL-2H3 MCs. A total of 949 lipid raft related proteins were discovered using two separate post-isolation treatment approaches. The bulk of these MC lipid raft proteins have already been documented in the RaftProtV2 database and are among the most frequently cited and experimentally validated lipid raft proteins. proteins. Furthermore, almost half of the proteins discovered showed lipid modifications and/or transmembrane domains. The proteins were shown to be connected with cellular membrane compartments and biological and molecular functions such as regulation, localisation, binding, catalytic activity, and stimulus response after they were classified into functional categories. Furthermore, functional enrichment analysis revealed that identified proteins are intimately involved in numerous areas of MC biological activities, particularly those related to controlled secretion, macromolecule complex organization/stabilization, and so on.

Author (s) Details

Edismauro Garcia Freitas Filho
Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP, zip code 14049-900, Brazil.

Luiz Augusto Marin Jaca
Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP, zip code 14049-900, Brazil.

Lilian Cristiane Baeza
Molecular Biology Laboratory, Biological Science Institute, Federal University of Goiás, Samambaia Campus II, ICB2, room 206, Goiânia, GO, zip code 74690-900, Brazil.

Célia Maria de Almeida Soares
Molecular Biology Laboratory, Biological Science Institute, Federal University of Goiás, Samambaia Campus II, ICB2, room 206, Goiânia, GO, zip code 74690-900, Brazil.

Clayton Luiz Borges
Molecular Biology Laboratory, Biological Science Institute, Federal University of Goiás, Samambaia Campus II, ICB2, room 206, Goiânia, GO, zip code 74690-900, Brazil.

Constance Oliver
Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP, zip code 14049-900, Brazil.

Maria Célia Jamur
Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP, zip code 14049-900, Brazil.

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