Developing a Stability Indicating Ultra Performance Liquid Chromatographic Method for Simultaneous Quantitation of Degradation Products of Telmisartan and Hydrochlorothiazide in Combination Dosage Form | Chapter 3 | Advanced Concepts in Pharmaceutical Research Vol. 9

 

Telmisartan (TLM) is an angiotensin receptor blocker (ARB) that has an extensive period of working and has the lengthy half-life of any ARB. Telmisartan works by blocking a substance in the body that causes blood vessels to tighten. This work is intended to thrive a stability-indicating Ultra performance liquid chromatographic method for the estimation of Telmisatam (TLM) and Hydrochlorothiazide (HCTZ) and degradation products pharmaceutical dosage forms. Separation was carried out on Zorbax Eclipse XDB C-18(50 x mm, 1.7 µm) column using a gradient method. Mobile phase A is 10mM KH2PO4 having 1% (v/v) of trimethylamine and mobile phase B is acetonitrile used in this work. 0.5 mL/ minute is the flow rate and at 271 nm noticed wavelength is monitored. Method development trails were carried out on six different columns. For specificity, limit of quantification, limit of detection, linearity, accuracy, method precision, robustness and stability this method is validated. The correlation coefficient of the impurities is more than 0.99. The stability indicating method confirmed that there was no interference of all impurities of TLM and HCTZ. TLM and HCTZ tablet samples stored and long-term storage conditions. The effect of storage conditions results proved that the method has stability indicating capability. Hence, the developed LC method was stability indicating and well applied for drug product stability study as well as for quality monitoring.

Author(s) Details:

Narasimha Reddy G. P,
Department of Chemistry, Sri Krihnadevaraya University Anantapur, Anantapuramu, Andhra Pradesh, India.

 

Sreenivasulu Reddy T.
Department of Chemistry, Sri Krihnadevaraya University Anantapur, Anantapuramu, Andhra Pradesh, India.


Sidda Reddy K.
Department of Chemistry, Sri Krihnadevaraya University Anantapur, Anantapuramu, Andhra Pradesh, India.

Shashi Kumar K. N.
Department of H&S, Annamacharya Institute of Technology & Sciences, Kadapa, 516003, Andhra Pradesh, India.


Please see the link here: https://stm.bookpi.org/ACPR-V9/article/view/14362

Occurrence of Endocrine-disrupting Herbicides and Related Multiresidues in Natural Surface and Ground Waters from Aquifer: Control at Regulatory Levels by a Validated and Robust SPE-RP-HPLC-DAD Screening Approach| Chapter 6 | Progress in Chemical Science Research Vol.3

 A dependable and powerful logical technique consolidating disconnected strong stage extraction (SPE) and turned around stage elite execution fluid chromatography (HPLC) with diode cluster location (DAD), has been created for the concurrent assurance of six endocrine-disruptor herbicides, in particular two parent S-triazines and their major dealkylated and hydroxylated debasement items like atrazine (AT), simazine (SI), deisopropyl-atrazine (DIA), deethyl-atrazine (DEA), hydroxyl-atrazine (H-AT) and hydroxyl-simazine (H-SI), every one of them with herbicide action as well. As ideal circumstances were chosen a Kromasil C18 section as fixed stage and a combination of acetonitrile and phosphate cradle (pH 7.0) as versatile stage in a slope elution mode and 215 nm as frequency for optical location.

A past SPE step with a polymeric cartridge (Bond-Elut ENV-1000 mg) was completed to disengage and preconcentrate the looked for analytes before chromatographic examination giving in this way a high enhancement variable of up to 3333. The streamlined disconnected SPE HPLC-DAD strategy was submitted to a broad approval convention acquiring among different boundaries, exceptionally delicate constraints of evaluation, somewhere in the range of 81 and 100 ng L-1, which were from ten to thirty-crease lower than the permitted maximal sums for regular surface waters (1 and 3 g L-1 as ready and alert levels), as per momentum European Union orders. The unwavering quality was surveyed by a comprehensive heartiness assessment in view of Plackett-Burman fragmentary factorial model followed by Youden and Steiner factual treatment. At last, the proposed technique was effectively applied to the screening of herbicides in ground and normal surface water tests from various areas in the 23th spring (Albacete, Spain), with exceptional concentration to the hydroxylated corruption items, extremely normal and whose location has been

barely tended to up until this point, regardless of their herbicide action as well, justification for why they become a significant matter of examination.

Author(s) Details:

María Jesús Villaseñor Llerena,
Department of Analytical Chemistry and Food Technology, Industrial Engineering School, University of Castilla-La Mancha, 13071, Ciudad Real, Spain.

Please see the link here: https://stm.bookpi.org/PCSR-V3/article/view/7965