Biopigment from Marine Soil Bacteria Citricoccus sp. | Chapter 2 | Science and Technology: Recent Updates and Future Prospects Vol. 10

Despite the complications in the isolation of pigments from natural resources, microbial pigments are increasingly gaining the attention of researchers because of their potential to be used in the textile, cosmetics, pharmaceuticals, food industries, etc. The aim of this study is to isolate, and characterize pigmented bacteria from marine soil and optimize the culture condition for optimum pigment production. The yellow-pigmented bacteria were isolated on a nutrient agar medium and screened for motility, gram staining, and biochemical tests.  The study mainly focused on using microbial pigment for dyeing cotton fabrics and woolen threads. We found out that the rate of pigment production was increased by optimizing the pH and temperature along with supplementing 1% glycerol as carbon and 0.5% beef extract as nitrogen sources. The pigment was extracted using methanol as solvent and was characterized through UV-visible spectroscopy and Fourier Transform Infrared (FTIR) spectroscopy whose values and phytochemical analysis results proved that the pigment belongs to the carotenoid group. Through 16s rRNA sequencing the bacteria was identified as Citricoccous sp.

 

 

Author(s) Details

D. Ezhil
Department of Biotechnology, M.S. Swaminathan Research Foundation, Chennai, Tamil Nadu, India.

S. Meignanalakshmi
Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai, Tamil Nadu, India.

B. Haritha
Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai, Tamil Nadu, India.

 

 

Please see the book here:- https://doi.org/10.9734/bpi/strufp/v10/2

 

Analysis of the Comparative Study on Flower Pigments with Phenylalanine Ammonia-Lyase Activity in Orchid Plants | Chapter 5 | Challenging Issues on Environment and Earth Science Vol. 3

 Plants in the Orchidaceae family have long been one of the most significant commercial products in the agricultural industry. The flower petals can be used as cut flowers as well as potted flowers because they have eye-catching pigments.

The main pigments found in orchid flower petals, as well as their relationships with phenylalanine ammonia-lyase (PAL) activity, were investigated.

The total anthocyanin content of six separate orchid petals was measured using spectrophotometry, and the values ranged from 0 mg/g (in Dendrobium Shavin white) to 2.128 mg/g (in Dendrobium Shavin red) (in Mokara Aranda). The petals with vibrant colours have a lot of anthocyanin, while those with pale colours have a lot of chlorophyll. When compared to -carotene and chlorophyll content, total anthocyanin content was found to be the highest. In a correlation study, PAL behaviour was found to be significantly positive correlated with anthocyanin material.

Conclusion: The findings suggest that the PAL enzyme may be used as a biomarker for orchid flower colour.

Author (s) Details

Suat Hian Tan
Faculty of Industrial Sciences and Tecehnology, College of Computing and Applied Sciences, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia.

Nyuk Ling Ma
School of Fundamental Science, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.

Wan Nurul Hidayah Wan Anuar
Faculty of Applied Sciences, Universiti Teknologi MARA, Perak Branch, Tapah Campus, Tapah Road, Malaysia.

View Book :- https://stm.bookpi.org/CIEES-V3/article/view/725