Development and Validation of a Simple and Reliable UV Spectrophotometric Method for the Simultaneous Estimation of Metformin Hydrochloride and Pravastatin Sodium | Chapter 9 | Pharmaceutical Science: New Insights and Developments Vol. 10

 

Background: Metformin hydrochloride is a drug used in the treatment of type 2 diabetes. It exhibits high aqueous solubility, limited solubility in ethanol, and negligible solubility in organic solvents such as acetone, ether, and chloroform.

 

Aim: A straightforward and reliable ultraviolet (UV) spectrophotometric method was developed and validated for the simultaneous estimation of Metformin Hydrochloride (MH) and Pravastatin Sodium (PS) in their pure forms.

 

Methodology: The proposed method employs an absorbance subtraction approach using UV spectrophotometry. Quantification was carried out by measuring absorbance at two selected wavelengths, 232 nm for Metformin Hydrochloride and 238 nm for Pravastatin Sodium. Method validation was performed in accordance with ICH guidelines, including accuracy studies conducted at three concentration levels (75%, 100%, and 125%), and percentage recovery was calculated for both drugs.

 

Results: The method demonstrated acceptable sensitivity, with limits of detection and quantification determined as 0.481 μg/mL and 0.670 μg/mL for MH, and 1.15 μg/mL and 1.68 μg/mL for PS, respectively. Statistical evaluation of validation parameters confirmed that the method exhibited satisfactory precision, accuracy, and selectivity within the specified limits.

 

Conclusion: The validated UV spectrophotometric method is simple, precise, and accurate, making it suitable for the simultaneous estimation of Metformin Hydrochloride and Pravastatin Sodium. The method can be effectively applied for routine analysis of these drugs in pure form and pharmaceutical dosage formulations.

 

 

Author(s) Details

Ankita Sharma
Shiva Institute of Pharmacy, Bilaspur, H.P., India.

 

Kapil Kumar Verma
Minerva College of Pharmacy, Indora, Kangra, H.P., India.

 

Inder Kumar
Minerva College of Pharmacy, Indora, Kangra, H.P., India.

 

Anju Bala
Chandigarh Group of Colleges Landran, Kharar, Greater Mohali, Punjab, India.

 

Bhumika Thakur
Shiva Institute of Pharmacy, Bilaspur, H.P., India.

 

Vandana Thakur
Abhilashi College of Pharmacy, Nerchowk, Mandi, H.P., India.

 

Please see the book here :- https://doi.org/10.9734/bpi/psnid/v10/7061

Study of Controlled Release of Ibuprofen Using Mg Al LDH Nano Carrier | Chapter 9 | New Approaches in Engineering Research Vol. 7

 NSAIDs (non-steroidal anti-inflammatory medicines) such as ibuprofen were intercalated in-situ into the interlayer gap of Mg Al LDH nanoparticles during the co-precipitation of hydroxides in the current study. LDHs are made up of a water molecule and a positive charge compensating and exchangeable anion. XRD, FTIR, and UV spectroscopy were used to characterise LDH nanohybrids. Co-precipitation was used to make Mg1-xAlx(NO3)x(OH)2.nH2O nanoparticles from an aqueous solution of Mg(NO3)2.6H2O and Al(NO3)3.9H2O. During the coprecipitation of the drug LDH conjugate in nitrogen, ibuprofen was intercalated in the interlayer gap of Mg-Al LDH. atmosphere. The nanopowders produced ranged in size from 25 to 90 nm, with an average particle size of 55 nm. Due to the intercalation of larger ibuprofen molecules in the interlayer space of LDH, XRD examination revealed a rise in d003 spacing from 7.89 for pure LDH to 14.71 for ibuprofen intercalated LDH. The intercalation of ibuprofen in the interlayer structure of LDH was confirmed by FTIR analysis, which revealed hydroxyl and carbonyl stretching of ibuprofen in the LDH-IBU sample. Using UV-Vis spectroscopy, a study of drug release kinetics in phosphate buffer solution at pH 7.4 revealed that 50% of drug molecules were released after 15 hours and more than 85% were released within 24 hours.

Author (s) Details

Dr. Sudip Dasgupta
Department of Ceramic Engineering, National Institute of Technology, Rourkela, Odisha 769008, India.

View Book :- https://stm.bookpi.org/NAER-V7/article/view/2228