Allelic Diversity at MHC-DQA Region in Indian Mithun (Bos Frontalis) | Chapter 3 | Contemporary Research and Perspectives in Biological Science Vol. 8

Background: Major histocompatibility complex (MHC) molecules are known to have a major role in immune response in all vertebrates. The genes encoding these MHC molecules are highly polymorphic and have always remained prime candidates for the investigation of genetic variation in the host resistance to infection in animals and humans. The genetic variation within this region also explains a substantial proportion of differential immune responsiveness between individuals. Aim: The present work was intended to identify new alleles of MHC-DQA genes and their diversity analysis in Mithun populations. This work is important for assessing genetic diversity in MHC DQA genes and is also helpful in generating an MHC allele database in mithun populations. Methodology: A Descriptive study was done at the Department of Animal Genetics and Breeding in ICAR–National Bureau of Animal Genetic Resources, Karnal, Haryana, India. between January 2015-April 2016. 79 Mithun blood samples were collected from different field areas of Nagaland and Arunachal Pradesh states of India. PCR-RFLP analysis was done by Hae III restriction enzyme further validation was done by sequencing. Results: In 79 mithuns from two Mithun populations (47 Nagami and 32 Arunachali type), a 776 nucleotide long genomic region comprising hyper variable exon 2 of Bofr-DQA was amplified. The area was then digested using the Hae III restriction enzyme for PCR-RFLP analysis. Few restriction patterns that carried the entire fragment size of the alleles aggregating more than a heterozygous state were found by the analysis. Conclusion: It was discovered that about half of the mithun population has a high polymorphism. Like other bovines, mithun may have a significant role in producing more diverse and abundant MHC class II molecules, which would give it the ability to connect with a greater number of pathogenic antigens. This will assist in creating haplo-groups and an MHC Allele database for Mithun populations.

 

Author (s) Details

 

Dimpee Singh Gonge
College of Veterinary Science and Animal Husbandry, Mhow (Indore), NDVSU Jabalpur, 482001, India.

 

S.K. Niranjan

ICAR–National Bureau of Animal Genetic Resources, Karnal, Haryana, 132001, India.

 

A. Upadhyay
Animal Husbandry and Dairy, College of Agriculture, Indore, 452001, India.

 

A. Suman
College of Veterinary Science and Animal Husbandry, Mhow (Indore), NDVSU Jabalpur, 482001, India.

 

K. Govil
College of Veterinary Science and Animal Husbandry, Rewa, NDVSU Jabalpur, 482001, India.

 

J. Yadav
College of Veterinary Science and Animal Husbandry, Mhow (Indore), NDVSU Jabalpur, 482001, India.

 

Please see the book here:- https://doi.org/10.9734/bpi/crpbs/v8/3532

Use of Molecular Methods for DNA Detection of Babesia canis vogeli in Blood Samples and Rhipicephalus sanguineus Ticks Collected in Dogs from Mexico | Chapter 11 | Newest Updates in Agriculture and Veterinary Science Vol. 1

 Three various subspecies of Babesia canis have been discovered on a global scale: B. canis canis in Europe, B. canis rossi in Africa, and B. canis vogeli, whose appearance has been habitual in America.  However, B. canis rossi has recently existed reported in the United States, and B. canis vogeli has currently been recognized in South Africa and Europe. The objective of this work search out optimize two forms for the molecular discovery of the B. canis subspecies causing dog babesiosis in Mexico. Canine babesiosis is reported as a worldly-wise tick-carried disease moving domestic dogs, and allure distribution is had connection with vector vicinity being more prevalent in domains with equatorial and subtropical climates.  Blood samples and Rhipicephalus sanguineus ticks, 30 blood samples, and 18 tick samples were taken from dogs accompanying clinical proofs compatible accompanying canine babesiosis and a record of exposure to ticks.  The reasoning of Restriction Fragment Length Polymorphisms in PCR-amplified and endured DNA with limit enzyme TaqI and HinfI (PCR-RFLP) admitted the detection of the matching pattern for the B. canis vogeli subspecies (DNA fragments of 203 bp, 171 bp and 26 bp) in two blood samples and individual of the tick specimens ticks liable to be subjected the PCR-RFLP analysis. Three ancestry samples and three of the ticks that were examined for the B. canis vogeli subspecies were raise to have the 192 bp fragment of interest using a reside PCR assay. Although B. canis's presence in Mexico has former been proved microscopically, this is the first report of the subspecies B. canis vogeli's microscopic detection in the country that has happened verified by DNA sequencing.

Author(s) Details:

Jose J. Lira-Amaya,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

Carmen Rojas-Martínez,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

J. Antonio Álvarez-Martínez,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

Alfredo Pelaez-Flores,
CENAPA–SENASICA–SADER, Carretera Cuernavaca Cuautla No 8534, Jiutepec, Morelos, C.P. 62550, México.

Francisco Martínez-Ibañez,
CENAPA–SENASICA–SADER, Carretera Cuernavaca Cuautla No 8534, Jiutepec, Morelos, C.P. 62550, México.

Diego Perez-de la Rosa,
CENAPA–SENASICA–SADER, Carretera Cuernavaca Cuautla No 8534, Jiutepec, Morelos, C.P. 62550, México.

Julio V. Figueroa-Millán,
CENID-Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla No. 8534, Jiutepec, Morelos, 62550, México.

Please see the link here: https://stm.bookpi.org/NUAVS-V1/article/view/8564